卵母细胞成熟度在高龄患者ICSI治疗结局中的影响研究  

Study on Effect of Oocyte Maturation on ICSI Outcomes in Elderly Patients

作　　者：谢丽松 周龙萍 岳林林 XIE Lisong;ZHOU Longping;YUE Linlin(不详;Department of Reproductive Medicine,Ganzhou Women and Children's Health Care Hospital,Ganzhou 341000,China)

机构地区：[1]赣州市妇幼保健院生殖科,江西赣州341000

出　　处：《中国医学创新》2024年第21期170-174,共5页Medical Innovation of China

基　　金：赣州市科技计划项目(GZ2021ZSF293)。

摘　　要：目的:探究高龄患者卵胞浆内单精子注射(ICSI)中卵母细胞成熟度对其治疗结局的影响。方法:选取赣州市妇幼保健院于2019年1月—2022年12月收治的进行ICSI助孕治疗的142例高龄患者作为研究对象,根据患者卵母细胞成熟度(成熟卵母细胞与取卵获得卵母细胞数之比)将患者分为A组(成熟度>80%,n=104)、B组(60%<成熟度≤80%,n=28)、C组(成熟度≤60%,n=10)。比较三组获卵情况及妊娠结局[受精率、双原核(2PN)受精率、优质胚胎率、囊胚形成率、累计临床妊娠率]。结果:三组获卵率、平均获卵数、≥14 mm卵泡数、MⅡ卵率比较,差异均有统计学意义(P<0.05)。A组获卵率、平均获卵数、≥14 mm卵泡数、MⅡ卵率均显著高于B组与C组(P<0.05)。三组受精率、优质胚胎率、囊胚形成率、累计妊娠率比较,差异均无统计学意义(P>0.05)。三组2PN受精率比较,差异有统计学意义,且以A组的2PN受精率最高(P<0.05)。A组与B组的累计妊娠率均高于C组,差异均有统计学意义(P<0.05)。结论:高龄患者行ICSI治疗结局受卵母细胞成熟度的影响,卵母细胞成熟度低可对获卵情况、受精及胚胎发育造成负面影响。Objective:To investigate the effect of oocyte maturation on intracytoplasmic sperm injection(ICSI)outcomes in elderly patients.Method:A total of 142 elderly patients who received ICSI assisted pregnancy in Ganzhou Women and Children's Health Care Hospital from January 2019 to December 2022 were selected as the research subjects,according to the degree of oocyte maturation(the ratio of mature oocytes to oocytes obtained),the patients were divided into group A(maturity>80%,n=104),group B(60%<maturity≤80%,n=28)and group C(maturity≤60%,n=10).Oocyte retrieval and pregnancy outcomes[fertilization rate,bipronuclear(2PN)fertilization rate,high-quality embryo rate,blastocyst formation rate and cumulative clinical pregnancy rate]were compared among the three groups.Result:There were statistically significant differences in oocyte retrieval rate,average number of retrieved oocytes,number of follicles≥14 mm and MⅡrate of oocytes among the three groups(P<0.05).The oocyte retrieval rate,average number of retrieved oocytes,number of follicles≥14 mm and MⅡrate of oocytes in group A were significantly higher than those in group B and group C(P<0.05).The fertilization rate,high-quality embryo rate,blastocyst formation rate and cumulative pregnancy rate showed no statistically significant differences among the three groups(P>0.05).The difference in fertilization rate of 2PN among the three groups was statistically significant,and 2PN fertilization rate in group A was the highest(P<0.05).The cumulative pregnancy rate of group A and group B was higher than that of group C,the differences were statistically significant(P<0.05).Conclusion:ICSI outcomes in elderly patients is influenced by oocyte maturation,and low oocyte maturation has adverse effect on oocyte retrieval,fertilization and embryonic development.

关 键 词：高龄 卵母细胞成熟度 卵胞浆内单精子注射 受精率 优质胚胎率 

分 类 号：R714.8[医药卫生—妇产科学]