具有凝血作用的原位快速凝胶化双醛淀粉/胶原蛋白水凝胶的制备  

Preparation of In-situ Rapid Gelation Dialdehyde Starch/Collagen Hydrogel with Coagulation Effect

作　　者：鲍宗源 欧天凯 谢新玲[1] 古文奇 张友全[1] BAO Zong-yuan;OU Tian-kai;XIE Xin-ling;GU Wen-qi;ZHANG You-quan(Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology,School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China)

机构地区：[1]广西石化资源加工及过程强化技术重点实验室,广西大学化学化工学院,南宁530004

出　　处：《高分子通报》2024年第8期1095-1107,共13页Polymer Bulletin

基　　金：国基自然科学基金(基金号22168011);广西石化资源加工及过程强化技术重点实验室主人基金(基金号2021Z011,2022Z003)。

摘　　要：为了改善胶原蛋白水凝胶在室温下的凝胶化速度,以双醛淀粉(DAS)和猪皮胶原蛋白为基料,制备了具有凝血作用的原位快速凝胶化双醛淀粉/胶原蛋白(DAS-Col)水凝胶。考察了DAS醛基含量和用量、胶原蛋白分子量和用量、Ca^(2+)浓度、pH值等对凝胶化时间的影响;表征了水凝胶的结构、杨氏模量、体外凝血指数(BCI)和全凝血时间。结果表明:醛基含量为33.48%、质量浓度为10 g/L的DAS溶液与相同质量浓度的胶原蛋白溶液按照体积比1:4的比例混合,在体系Ca^(2+)质量浓度为10 g/L、室温和pH=7.4条件下,凝胶时间短至93 s,杨氏模量达到143.1 kPa,BCI凝血指数为0.106,体外全凝血时间为152 s,呈现出良好的自行降解和自愈性能。具有庞大支链的DAS与胶原蛋白分子通过交联,形成庞大、交错的亲水性水凝胶网络骨架,是室温下快速形成水凝胶的关键。DAS-Col水凝胶是一种可以实现原位快速凝胶化的水凝胶,具有用于一些体表不规则伤口和体内深处伤口的止血护理的潜力。In order to improve the gelation speed of collagen hydrogel at room temperature,dialdehyde starch/collagen(DAS-Col)hydrogel with coagulation function was prepared based on DAS and pigskin collagen.The effects of DAS aldehyde group content and dosage,collagen molecular dosage,Ca^(2+)concentration and pH value on gelation time were investigated.The structure,Young’s modulus,coagulation index in vitro(BCI)and total coagulation time of the hydrogel were characterized.When a 10 g/L DAS solution with an aldehyde content of 33.48%and a 10 g/L collagen solution were mixed in a volume ratio of 1:4,Ca^(2+)concentration was 10 g/L,and the pH value was 7.4,the DAS-Col hydrogel was formed rapidly at room temperature,the gelation time was as short as 93 s,the Young’s modulus reached 143.1 kPa,the BCI was 0.106,and the total coagulation time in vitro was 152 s.Moreover,the DAS-Col hydrogel also exhibited excellent self-degradation and self-healing properties.The cross-linking of the DAS with huge branched chains and the collagen molecules would form a huge and staggered hydrophilic hydrogel network skeleton,which was the key to the rapid formation of hydrogel at room temperature.All the research results indicate that DAS-Col hydrogel will be a kind of hydrogel that can be rapidly gelled in situ,and it has the potential to be used for hemostasis care of some irregular wounds on the body surface and deep wounds in the body.

关 键 词：凝胶 生物质 蛋白质 双醛淀粉 止血 

分 类 号：TQ427.26[化学工程]