新疆和田地区鹅细小病毒感染调查及VP1基因遗传进化分析  

Investigation of Goose parvovirus infection and genetic evolution analysis of VP1 gene in Hotan region, Xinjiang Uygur Autonomous Region

作　　者：肖海侠 马万鹏 王燕 马雪连 米晓云[2] 刘黎 苏战强[1] XIAO Haixia;MA Wanpeng;WANG Yan;MA Xuelian;MI Xiaoyun;LIU Li;SU Zhanqiang(Xinjiang Agricultural University,Urumqi 835002,China;Institute of Veterinary Medicine,Xinjiang Academy of Animal Husbandry Sciences,Urumqi 835002,China;Hotan Animal Husbandry Technology Promotion Station,Hotan 848099,China)

机构地区：[1]新疆农业大学,乌鲁木齐835002 [2]新疆畜牧科学院兽医研究所,乌鲁木齐835002 [3]和田地区畜牧技术推广站,新疆和田848099

出　　处：《黑龙江畜牧兽医》2024年第14期68-73,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：和田地区特色畜禽安全高效生产关键技术集成与示范项目(2020B01004);2023年自治区现代畜禽种业提升专项(2023-xjjq-z-05)。

摘　　要：为了解新疆和田地区鹅细小病毒(Goose parvovirus,GPV)的感染情况及遗传进化特征,试验采集新疆和田地区和田县、于田县、皮山县鹅养殖场的578只死亡雏鹅的内脏(肝脏、脾脏)及肛拭子样本(和田县344只,于田县67只,皮山县167只),采用PCR方法对鹅细小病毒进行检测,并随机选取2份阳性病料样本对鹅细小病毒VP1基因进行PCR扩增、测序、BLAST比对和遗传进化分析。结果表明:共检测出44份鹅细小病毒阳性样品,总阳性率为7.61%(44/578),其中新疆和田地区和田县鹅细小病毒阳性率为12.50%(43/344),于田县鹅细小病毒阳性率为1.49%(1/67),皮山县未检测到鹅细小病毒。随机选取的2株鹅细小病毒(XJHT-1、XJHT-2株)均为经典鹅细小病毒,与鹅细小病毒各参考毒株VP1基因编码氨基酸序列相似性分别为89.5%~95.8%和92.1%~97.3%,与番鸭细小病毒参考毒株VP1基因编码氨基酸序列相似性分别为76.0%~85.4%和78.5%~88.7%。鹅细小病毒XJHT-1、XJHT-2株与鹅细小病毒DY16株(中国江苏)的相似性最高,分别为95.8%和97.3%,遗传距离最近;XJHT-1株与鹅细小病毒GD株(中国广东)和SD-F30株(中国河北)的相似性较低,分别为89.5%和89.6%;XJHT-2株与鹅细小病毒SHM319株(德国)、GD株(中国广东)和SD-30株(中国河北)的相似性较低,分别为92.1%、92.8%和92.8%。说明和田地区部分鹅场存在鹅细小病毒的感染,但阳性率较低,选取的2株毒株与鹅细小病毒DY16株(中国江苏)的氨基酸序列相似性最高,推测可能由鹅细小病毒DY16株进化而来。In order to understand the infection situation and genetic evolution characteristics of Goose parvovirus(GPV) in Hotan area of Xinjiang Uygur Autonomous Region, in the experiment, viscera(liver and spleen) and anal swabs of dead goslings were collected from 578 geese of goose farms in Hotan, Yutian and Pishan Counties in Hotan region of Xinjiang(344 geese from Hotan County, 67 geese from Yutian County, and 167 geese from Pishan County). The PCR method was used to detect Goose parvovirus;and two positive samples of diseased materials from Hotan County, Hotan region were randomly selected for PCR amplification and genetic evolution analysis of Goose parvovirus VP1 gene. The results showed that a total of 44 positive samples of Goose parvovirus were detected, with an overall positive rate of 7.61%(44/578);among them, the positive rate of Goose parvovirus in Hotan County of Hotan region, Xinjiang Uygur Autonomous Region was 12.50%(43/344), the positive rate of Goose parvovirus in Yutian County was 1.49%(1/67), and no Goose parvovirus was detected in Pishan County. Two random strains of goose parvovirus(XJHT-1 and XJHT-2 strains) were classical goose parvovirus.The amino acid sequence similarity of VP1 gene between the two strains of Goose parvovirus and each reference strain of Goose parvovirus, ranged from 89.5% to 95.8% and 92.1% to 97.3%, respectively, with the reference strain of Duck parvovirus VP1 gene, ranging from 76.0% to 85.4% and 78.5% to 88.7%, respectively. Among them, XJHT-1 and XJHT-2 had the highest similarity with Goose parvovirus DY16(Jiangsu, China) with the closest genetic distance of 95.8% and 97.3%, respectively;XJHT-1 had lower similarity with Goose parvovirus GD(Guangdong, China) and SD-F30(Hebei, China) with 89.5% and 89.6%, respectively;XJHT-2 showed low similarity with Goose parvovirus SHM319(Germany), GD(Guangdong, China) and SD-F30(Hebei, China), with 92.1%, 92.8% and 92.8%, respectively. The results indicated that the infection of Goose parvovirus existed in some goose farms in Hotan reg

关 键 词：和田地区 鹅细小病毒 流行病学调查 VP1基因 遗传进化分析 

分 类 号：S835[农业科学—畜牧学]