pBD-2对热应激致小鼠小肠形态结构损伤及紧密连接蛋白表达的修复作用  

Repairing effects of pBD-2 on heat stress-induced morphological and structural damage and tight junction protein expression in mouse small intestine

作　　者：汤雨晴 张曼 齐振昌 李庆豪 李艺雷 董涵方 孙娟 金鑫 TANG Yuqing;ZHANG Man;QI Zhenchang;LI Qinghao;LI Yilei;DONG Hanfang;SUN Juan;JIN Xin(College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,China;College of Veterinary Medicine,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;Fuwai Central China Cardiovascular Hospital,Zhengzhou 451464,China)

机构地区：[1]河南农业大学动物医学院,郑州450046 [2]河南牧业经济学院动物医药学院,郑州450046 [3]阜外华中心血管病医院,郑州451464

出　　处：《黑龙江畜牧兽医》2024年第14期118-123,128,129,共8页Heilongjiang Animal Science And veterinary Medicine

基　　金：河南农业大学博士启动金项目(30500750)。

摘　　要：为了研究猪β-防御素-2(porcinβ-defensin2,pBD-2)对热应激致小鼠小肠形态结构损伤及紧密连接蛋白表达的修复作用,试验将36只1月龄昆明小鼠随机分为空白对照组、热应激组和热应激+pBD-2组,每组12只。空白对照组和热应激组每天每只小鼠按体重10μL/g以灌胃的方式给予生理盐水,热应激+pBD-2组每天每只小鼠按体重0.01μg/g以灌胃的方式给予pBD-2。灌胃后将热应激组和热应激+pBD-2组放入40℃温箱热应激处理1 h,取出后与空白对照组一起放置于室温下进行饲养。按照相同的方式共处理28 d,并于第28天处理后断颈处死。每天灌胃前观察各组的行为,记录采食量并称重,试验结束后计算各组平均日采食量和平均日增重,并对小鼠进行剖检,分别取十二指肠、空肠、回肠组织进行石蜡切片制作和H.E.染色,比较各组肠绒毛高度与隐窝深度比值和单位面积内杯状细胞数目,并采用免疫组织化学技术对紧密连接蛋白ZO-1、Claudin-3和Occludin进行检测与相对表达量分析。结果表明:试验期间,空白对照组无异样行为;热应激组探索行为增加,焦虑好动;热应激+pBD-2组比热应激组安静。热应激组平均日采食量显著高于热应激+pBD-2组和空白对照组(P<0.05),而热应激+pBD-2组和空白对照组间差异不显著(P>0.05)。空白对照组和热应激+pBD-2组平均日增重显著高于热应激组(P<0.05),空白对照组和热应激+pBD-2组平均日增重差异不显著(P>0.05)。空白对照组肠道健康、无红肿,肠道内容物适中;热应激组肠道表面充血、红肿,肠道内容物较多;热应激+pBD-2组肠道无红肿现象,肠道内容物较少。热应激组十二指肠、空肠、回肠绒毛高度与隐窝深度比值均显著低于空白对照组和热应激+pBD-2组(P<0.05),空白对照组和热应激+pBD-2组间差异不显著(P>0.05)。热应激+pBD-2组十二指肠和空肠单位面积内杯状细胞数量显著高于空白对照组�In order to investigate the repairing effects of porcinβ-defensin-2(pBD-2)on heat stress-induced morphological and structural damage and expression of tight junction proteins in mouse small intestine,in the experiment,thirty-six 1-month-old Kunming mice were randomly divided into a blank control group,a heat stress group,and a heat stress+pBD-2 group;with 12 mice in each group.The blank control group and heat stress group were given saline by gavage at 10μL/g of body weight per mouse per day;the heat stress+pBD-2 group was given pBD-2 by gavage at 0.01μg/g of body weight per mouse per day.After gavage,the heat stress group and the heat stress+pBD-2 group were placed in a 40℃incubator for 1 h.After removal,they were kept at room temperature together with the blank control group.The groups were treated in the same way for a total of 28 d,and were killed by breaking their necks on the 28th day of treatment.The behavior of each group was observed before gavage every day,and the feed intake was recorded and weighed;at the end of the experiment,the average daily feed intake and average daily weight gain of each group were calculated;and the abdominal cavity of the mice was dissected.Duodenum,jejunum and ileum tissues were taken for paraffin section making and H.E.staining respectively,to compare the ratio of height of intestinal villi to depth of crypts and the number of goblet cells per unit area in each group;and the tight junction proteins,ZO-1,Claudin-3 and Occludin,were detected by immunohistochemistry and analyzed for their relative expression.The results showed that during the trial,the blank control group had no unusual behavior;the heat stress group had increased exploratory behavior and was anxious and active;and the heat stress+pBD-2 group was quieter than the heat stress group.The average daily feed intake was significantly higher in the heat stress group than in the heat stress+pBD-2 group and the blank control group(P<0.05),while the difference between the heat stress+pBD-2 group and the blank contro

关 键 词：猪β-防御素-2(pBD-2) 热应激 小肠 形态结构 紧密连接蛋白 

分 类 号：S859.1[农业科学—临床兽医学]