拟青霉属内生真菌对杞柳耐盐性的影响  

Effects of endophytic fungi(Paecilomyces sp.)on the salt tolerance of Salix integra

作　　者：季文惠 谢东青 钟非[1,2] 朱蓉 胡颖 陈艳红 刘国元[1,2] 张健 JI Wenhui;XIE Dongqing;ZHONG Fei;ZHU Rong;HU Ying;CHEN Yanhong;LIU Guoyuan;ZHANG Jian(School of Life Science,Nantong University,Nantong 226019,China;Key Laboratory of Landscape Plant Genetics and Breeding,Nantong 226019,China)

机构地区：[1]南通大学生命科学学院,南通226019 [2]南通市观赏植物遗传育种重点实验室,南通226019

出　　处：《应用与环境生物学报》2024年第3期521-529,共9页Chinese Journal of Applied and Environmental Biology

基　　金：江苏省重点研发项目(BE2022420);江苏省林业科技创新与推广项目(LYKJ[2023]02);南通市社会民生项目(MS12021066,MS12022028)资助。

摘　　要：滩涂盐碱地是重要的后备土地资源,其改良与开发利用具有重要意义;生物改良法是改善盐碱地土壤质量的有效手段之一,其核心是如何提高植物对盐渍环境的适应性.将一株从海岸带植物根系分离的拟青霉属内生真菌(Paecilomyces sp.)接种到适应轻度盐渍环境的杞柳(Salix integra)幼苗根系,探讨该菌株提高杞柳耐盐性的潜力及相关机制.通过比较接种拟青霉属菌株对杞柳生长、生理与光合指标的影响,发现在盐胁迫条件下,处理组(接种内生真菌)杞柳根长、根系表面积与体积显著高于对照组(分别增加了163.4%、72.4%和96.4%);叶片过氧化氢酶(CAT)活性、生长素(IAA)含量和主要叶绿素荧光参数(光系统Ⅱ实际光化学量子效率ΦPSⅡ、光化学淬灭系数qP与光合电子传递效率ETR)均显著增加(P<0.05).在盐胁迫条件下,从接种与未接种拟青霉属菌株的杞柳叶片中共鉴定出701个差异表达基因(DEGs),主要富集于苯丙烷类生物合成、植物?病原体互作、植物激素信号转导以及淀粉与蔗糖代谢等通路其中,上调表达的基因显著富集于植物激素信号转导通路.基因功能分析表明,注释到茉莉酸、乙烯、生长素与细胞分裂素信号转导和油菜素甾醇生物合成通路的基因在拟青霉属菌株调控杞柳耐盐性中发挥重要作用.综上,该株拟青霉属内生真菌可有效缓解盐胁迫对杞柳幼苗的不利影响.The rehabilitation and reclamation of coastal saline-alkali land is of great significance since it is regarded as a reserve resource for cultivated land.Biological soil amelioration is an effective means of improving soil quality in saline-alkali land and focuses on improving the adaptability of plants to saline environments.In this study,an endophytic fungus(Paecilomyces sp.)isolated from the roots of a coastal plant was inoculated into the roots of Salix integra seedlings.The potential of this strain to promote plant growth under salt stress was explored,and the relevant mechanisms are discussed.The effects of inoculation with Paecilomyces sp.on the growth,physiology,and photosynthetic indices of Salix integra under salt stress were compared.Gene expression differences in the leaves of Salix integra before and after inoculation with Paecilomyces sp.were analyzed.Compared with the control group without inoculation,root length,root surface area,and root volume of Salix integra in the treatment group(inoculated with Paecilomyces sp.)were significantly increased by 163.4%,72.4%and 96.4%,respectively;the catalase(CAT)activity,indole-3-acetic acid(IAA)concentration,and core chlorophyll fluorescence parameters(photosystemⅡactual photochemical quantum efficiency(ΦPSⅡ),photochemical quenching coefficient(qP),and electron transportation rate(ETR))in leaves were significantly enhanced(P<0.05).Under salt stress,701 differentially expressed genes(DEGs)were identified in the leaves of Salix integra before and after endophytic fungus inoculation,according to transcriptome data.They were mainly enriched in phenylpropane biosynthesis,plant-pathogen interactions,plant hormone signal transduction,starch/sucrose metabolism,and other pathways.Among the Kyoto Encyclopedia of Genes and Genome(KEGG)pathways,the upregulated DEGs were significantly enriched in plant hormone signaling pathways.Gene function analysis indicated that genes annotated in the jasmonic acid-,ethylene-,auxin-,and cytokinin signaling and brassinol biosynthes

关 键 词：内生真菌 杞柳 盐胁迫 生长指标 转录组分析 植物激素 

分 类 号：S564.5[农业科学—作物学]