基于COⅠ和ITS2及16S rDNA基因序列对盾糙璃眼蜱的分子分类与厘定  

Molecular taxonomic and categorization of Hyalomma scupense based on COⅠ,ITS2 and 16S rDNA genes

作　　者：王彦龙 罗金[2] 车丽妍 昝晓庆 朱婷婷 赵爱云[1] 任巧云[2,3] 张振杰 刘光远 齐萌[1] WANG Yanlong;LUO Jin;CHE Liyan;ZAN Xiaoqing;ZHU Tingting;ZHAO Aiyun;REN Qiaoyun;ZHANG Zhenjie;LIU Cuangyuan;QI Meng(College of Animal Science and Technology,Tarim University,Aral 843300,China;State Key Laboratory for Animal Disease Control and Prevention/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Open Laboratory of Herbivores Animal Epidemics,Ministry of A griculture/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;College of Life Sciences,Hebei Normal University,Shijiazhuang 050024,China;School of A griculture,Ningxia University,Yingchuan 750021,China)

机构地区：[1]塔里木大学动物科学与技术学院,新疆阿拉尔843300 [2]中国农业科学院兰州兽医研究所动物疫病防控全国重点实验室甘肃省动物寄生虫病重点实验室农业部草食动物疫病重点开放实验室,甘肃兰州730046 [3]宁夏大学农学院,宁夏银川750021 [4]河北师范大学生命科学学院,河北石家庄050024

出　　处：《中国兽医科学》2024年第7期930-936,共7页Chinese Veterinary Science

基　　金：国家寄生虫资源库项目(NPRC-2019-194-30);中国农业科学院科技创新工程项目(ASTIP);国家肉牛牦牛产业技术体系项目(CARS-37);甘肃省基础研究创新群体项目(22JR5RA024);新疆生产建设兵团第九师科技计划项目(2023JS002)。

摘　　要：盾糙璃眼蜱的分类尚不明确,为厘清盾糙璃眼蜱的分类,解析其系统发育关系,本研究自新疆维吾尔自治区莎车县采集动物体表寄生的蜱,对其进行形态学观察,初步鉴定蜱种类;提取蜱的DNA样本后,基于蜱的COⅠ、ITS2和16SrDNA基因,对DNA样本进行PCR扩增、序列分析和种类鉴定,利用MEGA7.0软件构建系统发育树。结果显示,经形态学观察,初步鉴定本研究所获蜱的种类为盾糙璃眼蜱;将测序获得的COⅠ、ITS2和16S rDNA基因序列进行比对和同源性分析,发现与国内外已鉴定的盾糙璃眼蜱序列同源性分别为99.84%、99.52%和98.42%,鉴定本研究所获蜱的种类为盾糙璃眼蜱。种系发育树显示,本研究所获蜱的COⅠ、ITS2和16SrDNA序列均与已知盾糙璃眼蜱的序列聚成一支,与璃眼蜱属蜱的序列聚类形成一个大支,而与其他蜱种类形成不同分支。本研究结果为新疆部分地区蜱种分布和鉴定提供了基础参考数据。Classification of Hyalomma scuppense is still unclear.In order to clarify the classification of Hyalomma scupense and analyze its phylogenetic relationships.In this study,ticks parasitizing on animal surface were collected from Shache County,Xinjiang Uygur Autonomous Region,morphological observation was carried out to identify the species of ticks.After extracting DNA samples from ticks,PCR,sequence analysis,and species identification were performed on the DNA samples based on the COⅠ,ITS2,and 16S r DNA genes of ticks.A phylogenetic tree was constructed using MEGA 7.0.The results showed that the species of tick obtained in this study was preliminarily identified as Hyalomma scuppense by morphological observation.The sequences of COⅠ,ITS2,and 16S r DNA gene were compared with Hyalomma scuppense sequences that have been identified both domestically and internationally and homologous analysis was performed.The sequence homology was 99.84%,99.52%and 98.42%,respectively,and the species of ticks obtained in this study was identified as Hyalomma Scutella.Phylogenetic trees showed that the COⅠ,ITS2,and 16S r DNA sequences of the ticks obtained in this study were first clustered into a branch with the known Hyalomma scuppense sequences,and forming a large branch with the Hyalomma tick sequencs,and forming different branches from other tick species.The research results provide basic reference data for the distribution and identification of tick species in some areas of Xinjiang.

关 键 词：盾糙璃眼蜱 分类 形态学 分子鉴定 系统发育 

分 类 号：S852.746[农业科学—基础兽医学]