大肠杆菌表达动物病毒样颗粒疫苗的研究进展  

Research progress of animal virus-like particles vaccine expressed by Escherichia coli

作　　者：李幽幽 扶星星 朱玲[1] 王永胜 吴福文 张海[2] 徐志文[1] LI Youyou;FU Xingxing;ZHU Ling;WANG Yongsheng;WU Fuwen;ZHANG Hai;XU Zhiwen(Animal Biotechnology Center,Sichuan Agricultural University,Chengdu 611100,China;College of Bioengineering,Sichuan Water Conservancy Vocational College,Chengdu 611230,China;Sichuan Bainuoji Technology Co.,Ltd.Mianyang621000,China)

机构地区：[1]四川农业大学动物生物技术中心,四川成都611100 [2]四川水利职业技术学院生物工程学院,四川成都611230 [3]四川百诺吉科技有限公司,四川绵阳621000

出　　处：《中国兽医科学》2024年第7期955-961,共7页Chinese Veterinary Science

基　　金：国家农业产业技术体系四川兽药创新团队专项(CARS-SVDIP);四川省“十四五”川猪重大科技专项(2021ZDZX0010-3)。

摘　　要：大肠杆菌表达宿主因其表达量高、周期短、成本低等诸多优势而被广泛用作异源蛋白质的表达。据统计,超过30%的重组蛋白质药物和50%重组蛋白质的制备是使用大肠杆菌作为表达宿主的。大肠杆菌是低成本表达重组蛋白的首选生物之一,这对于兽用疫苗的生产尤其重要。本文系统阐述了大肠杆菌表达系统在动物病毒样颗粒疫苗应用中的生产和研究进展,并且总结了大肠杆菌表达系统在疫苗应用中存在的问题及现有的解决策略,以期为以大肠杆菌作为表达宿主的动物病毒样颗粒疫苗研制提供参考。The Escherichia coli expression system is widely employed for heterologous protein expression due to its numerous advantages,including high expression yield,short production cycle,and low cost.It has been reported that more than 30%of recombinant protein drugs and 50%of recombinant protein preparations utilize Escherichia coli as the preferred host organism.consequently,Escherichia coli holds significant importance in the economical production of veterinary vaccines.This paper provides a comprehensive overview of the production and research advancements in utilizing the Escherichia coli expression system for animal virus-like particle vaccine applications.Furthermore,it summarizes the current challenges faced by this system in vaccine development along with potential solutions,aiming to offer valuable insights for future studies on animal virus-like particle vaccines employing Escherichia coli as an expression host.

关 键 词：大肠杆菌 表达宿主 病毒样颗粒疫苗 

分 类 号：S852.65[农业科学—基础兽医学]