新疆南疆地区116株结核分枝杆菌基因分型研究  

Genotyping of 116 strains of Mycobacterium tuberculosis in Southern Xinjiang

作　　者：朱婷婷 王乐[2] 阿依努尔·莫合买提[2] 祖丽卡提阿衣·阿布都拉 王明哲 古丽娜·巴德尔汗[2] 王森路 刘万里[2] ZHU Tingting;WANG Le;Ayinuer Mohemaiti;Zulikatiayi Abudula;WANG Mingzhe;Gulina Badeerhan;WANG Senlu;LIU Wanli(School of Public Health,Xinjiang Medical University,Urumqi,Xinjiang 830054,China;Xinjiang Center for Disease Control and Prevention,Urumqi,Xinjiang,830002,China)

机构地区：[1]新疆医科大学公共卫生学院,新疆乌鲁木齐830054 [2]新疆维吾尔自治区疾病预防控制中心,新疆乌鲁木齐830002

出　　处：《中国热带医学》2024年第7期826-832,共7页China Tropical Medicine

基　　金：新疆维吾尔自治区自然科学基金项目(No.2023D01C57);新疆维吾尔自治区科学技术协会青年人才托举工程项目(No.RCTJ42);新疆维吾尔自治区卫生健康青年医学科技人才专项科研项目(No.WJWY-202347)。

摘　　要：目的探讨新疆维吾尔自治区南疆地区结核分枝杆菌(Mycobacterium tuberculosis,MTB)分子流行病学特点,分析MTB菌株成簇的影响因素,为新疆南疆地区结核病防治提供科学依据。方法随机抽取2018年和2020—2022年新疆南疆地区耐药监测点的结核分枝杆菌,提取菌株DNA,采用24位点可变串联重复序列分型方法对菌株进行基因分型,对结核分枝杆菌进行位点多样性及分辨力检测,分析新疆南疆地区菌株的成簇率并比较其传播差异。结果116株结核分枝杆菌分90个基因型,其中独立基因型76种,其余的菌株成14簇,每簇2~7株,共40株,成簇率34.48%,传播率22.41%。分辨指数最高的为QUB-11b位点,分辨力指数为0.801;分辨指数最低的位点是MIRU2,分辨力指数为0。北京基因型在新疆南疆地区结核分枝杆菌中占44.83%(52/116)。菌株是否成簇与是否异烟肼耐药、利福平耐药差异有统计学意义(χ^(2)=6.798,P=0.009;χ^(2)=15.544,P<0.001)。结论新疆南疆地区结核分枝杆菌基因型呈高度多态性,但北京基因型仍是优势菌株,新疆南疆地区结核分枝杆菌的成簇率较高,这提示近期传播在新疆南疆地区结核病的流行中占有一定比例。新疆南疆地区北京基因型相对于非北京基因型、利福平耐药菌株相对于非利福平耐药菌株、乙胺丁醇耐药菌株相对于非乙胺丁醇耐药菌株更容易成簇。Objective To investigate the molecular epidemiological characteristics of Mycobacterium tuberculosis in the southern region of Xinjiang,analyze the influencing factors of Mycobacterium tuberculosis strains clustering in the region,and provide a scientific basis for the prevention and treatment of tuberculosis in the southern region of Xinjiang.Methods Mycobacterium tuberculosis strains were randomly selected from the drug resistance monitoring sites in the southern region of Xinjiang Uygur Autonomous Region from January 2018 to December 2022(except 2019).The DNA of the strains was extracted,and the strains were genotyped by the 24-locus VNTR method.The diversity and discriminatory power of loci were assessed,and the cluster rate and transmission differences of strains in Southern Xinjiang were analyzed.Results A total of 116 Mycobacterium tuberculosis strains were divided into 90 genotypes,including 76 unique genotypes,and the remaining strains formed 14 clusters of 2-7 strains each,totaling 40 strains,with a cluster formation rate of 34.48%and a transmission rate of 22.41%.The highest resolution index was observed at the QUB-11b(0.801)locus,with a discriminatory index of 0.801,while the lowest was at the MIRU2 locus,with a discriminatory index of 0.Beijing genotype accounted for 44.83%(52/116)of the MTB strains in southern Xinjiang.Statistical significance was found between strain clustering and resistance to isoniazid and rifampicin(χ^(2)=6.798,P=0.009;χ^(2)=15.544,P<0.001).Conclusions The genotypes of Mycobacterium tuberculosis in southern Xinjiang are highly polymorphic,but the Beijing genotype remains the predominant strain.The clustering rate of Mycobacterium tuberculosis in southern Xinjiang is higher,suggesting that the recent transmission of Mycobacterium tuberculosis in southern Xinjiang accounts for a certain proportion of the epidemic.In southern Xinjiang,Beijing genotype,rifampicin-resistant strains,and ethambutol-resistant strains are more likely to be clustered than non-Beijing genotype,rifampici

关 键 词：结核分枝杆菌 可变串联重复序列 基因分型 成簇率 

分 类 号：R378.911[医药卫生—病原生物学]