析因设计在蝇类现场诱捕试验中的应用  

Application of factorial design in the field trapping experiment of flies

作　　者：李炳辉 朱海龙[1,2] 李松 付齐齐 马小芳 刘砚涛[1,2] 孙钦同 LI Binghui;ZHU Hailong;LI Song;FU Qiqi;MA Xiaofang;LIU Yantao;SUN Qintong(Qingdao Municipal Center for Disease Control and Prevention,Qingdao,Shandong 266000,China;Qingdao Institute of Preventive Medicine,Qingdao,Shandong 266000,China;Jimo District Health Service Center,Qingdao,Shandong 266000,China;Shandong Provincial Center for Disease Control and Prevention,Ji'nan,Shandong 250000,China)

机构地区：[1]青岛市疾病预防控制中心,山东青岛266000 [2]青岛市预防医学研究院,山东青岛266000 [3]青岛市即墨区卫生健康事业服务中心,山东青岛266000 [4]山东省疾病预防控制中心,山东济南250000

出　　处：《中国热带医学》2024年第7期875-879,共5页China Tropical Medicine

基　　金：2021年度山东省人文社会科学课题项目(No.2021-ZXJK-17)。

摘　　要：目的探讨析因设计在蝇类现场诱捕试验中的应用,为筛选最佳灭蝇方式提供更多思路。方法采用2×2析因设计研究诱蝇笼放置高度(悬挂式诱蝇笼、立式诱蝇笼)和诱饵类型(商品诱饵、臭豆腐)2个因素的主效应及二者之间的交互作用。现场捕蝇方式共分为4种组合,分别为悬挂式诱蝇笼和商品诱饵、悬挂式诱蝇笼和臭豆腐、立式诱蝇笼和商品诱饵、立式诱蝇笼和臭豆腐,每种组合重复试验7次,诱捕试验参照《全国病媒生物监测实施方案》进行。通过析因设计方差分析法筛选最佳蝇类诱捕方式。结果本次研究共诱捕蝇类2296只,现场累计布放28笼,平均蝇密度为82.0只/笼。其中,立式诱蝇笼和臭豆腐组合诱蝇数量最多,占总捕蝇数的44.16%(1014/2296),平均蝇密度144.8只/笼;其次是悬挂式诱蝇笼和臭豆腐组合、悬挂式诱蝇笼和商品诱饵组合、立式诱蝇笼和商品诱饵组合,分别占42.73%(981/2296)、8.71%(200/2296)、4.40%(101/2296),平均蝇密度依次为140.1、28.6、14.4只/笼。不同诱捕方式现场诱蝇密度差异有统计学意义(F=49.441,P<0.001),诱蝇种群构成差异有统计学意义(χ^(2)=259.980,P<0.001)。诱蝇笼放置高度的主效应不显著(F=0.223,P=0.641),诱饵类型主效应显著(F=147.206,P<0.001),诱蝇笼放置高度和诱饵类型的交互作用不显著(F=0.894,P=0.354)。结论析因设计相较于单因素设计方法不仅能分析各因素的主效应,还能分析各因素间的交互作用,优选立式诱蝇笼和臭豆腐组合灭蝇效果好。Objective To explore the application of factorial design in field trapping experiments for flies,and to provide more ideas for screening the best fly control method.Methods A 2×2 factorial design was used to analyze the main effects of the height of the fly cage(hanging fly cage,standing fly cage)and the type of bait(commercial bait,smelly tofu bait),and the interaction between the two factors.The fly trapping methods were divided into four combinations:hanging fly cage and commercial bait,hanging fly cage and smelly tofu bait,standing fly cage and commercial bait,standing fly cage and smelly tofu bait.The experiment was repeated 7 times for each combination and carried out according to the National Vector Biological Surveillance Implementation Plan.The best fly trapping method was identified by factorial design analysis of variance.Results A total of 2296 flies were trapped in this study,with a cumulative deployment of 28 traps in the field,resulting in an average total density of 82.0 flies per cage.Among them,the number of flies attracted by the combination of standing fly cage and smelly tofu was the largest,accounting for 44.16%(1014/2296)of the total number of flies caught,with an average fly density of 144.8 flies per trap.This was followed by the combination of hanging fly cage and smelly tofu,hanging fly trap and commercial bait,and standing fly cage and commercial bait,accounting for 42.73%(981/2296),8.71%(200/2296),and 4.40%(101/2296)of the total,with average fly densities of 140.1,28.6,and 14.4 flies per trap,respectively.The differences in field fly density among different trapping methods were statistically significant(F=49.441,P<0.001),and the differences in fly population composition were also statistically significant(χ^(2)=259.980,P<0.001).The main effect of trap height was not significant(F=0.223,P=0.641),while the main effect of bait type was significant(F=147.206,P<0.001),and the interaction effect between trap height and bait type was not significant(F=0.894,P=0.354).Conclusions Compared w

关 键 词：媒介生物 蝇类 析因设计 多因素设计 单因素设计 诱蝇笼 

分 类 号：R184.33[医药卫生—流行病学]