力竭运动对大鼠肾功能和肾组织紧密连接蛋白的影响  

The influence of exhaustive exercise on renal function and renal tight junction protein in rats

作　　者：聂梦俭 李方 任盼红 Nie Mengjian;Li Fang;Ren Panhong(School of Physical Education,Henan Normal University,Xinxiang 453007,China;Department of Physical Education,Sanquan College of Xinxiang Medical University,Xinxiang 453003,China)

机构地区：[1]河南师范大学体育学院,河南新乡453007 [2]新乡医学院三全学院体育部,河南新乡453003

出　　处：《河南师范大学学报（自然科学版）》2024年第5期142-149,共8页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家社科基金(22BTY091);河南省重点研发与推广专项(232102321127);河南省高等学校智库研究项目(2024ZKYJ09).

摘　　要：[目的]探讨大强度力竭运动致蛋白尿负荷大鼠肾功能和肾组织紧密连接蛋白的变化及意义,为运动性肾损伤的防治及其机制研究提供新的思路.[方法]SD雄性大鼠18只,其中8只设为正常对照组,10只为力竭运动组,建立4周大强度力竭训练模型,造成大鼠持续的运动性蛋白尿负荷;检测肾功能血液指标即血清肌酐(Cre)和血尿素氮(BUN),检测肾损伤尿液指标即尿中性粒细胞明胶酶相关脂质运载蛋白(NGAL)和肾损伤分子1(KIM-1)水平变化;苏木青-伊红(HE)染色观察大鼠肾组织病理损伤情况;采用Westernblot及免疫组化方法检测肾组织中紧密连接蛋白1(ZO-1)和密封蛋白Claudin-1的表达变化.[结果]4周大强度力竭运动可导致大鼠运动性蛋白尿负荷,上调肾功能标志物血清Cre(P<0.01)和BUN(P<0.01),尿NGAL(P<0.01)和KIM-1(P<0.01)水平,下调ZO-1(P<0.01)、Claudin-1(P<0.05)在蛋白水平的表达.[结论]4周力竭运动可造成大鼠持续的运动性蛋白尿负荷,并导致大鼠运动性肾损伤,长时间力竭运动可能通过下调肾脏紧密连接蛋白而影响肾小管上皮紧密连接结构的完整性.[Objective]To investigate the changes and significance of renal function and tight junction proteins in rats with high-intensity exhaustive exercise-induced proteinuria,providing new insights for the prevention and treatment of exercise-induced kidney injury and its mechanism study.[Methods]Eighteen male SD rats were divided into a normal control group(n=8)and an exhaustive exercise group(n=10).A 4-week high-intensity exhaustive training model was established to induce sustained exercise-induced proteinuria in rats.Blood indicators of renal function,including serum creatinine(Cre)and blood urea nitrogen(BUN),as well as urine indicators of renal damage,such as neutrophil gelatinase-associated lipocalin(NGAL)and kidney injury molecule 1(KIM-1)levels,were measured.Hematoxylin-eosin(HE)staining was performed to observe pathological changes in rat kidney tissues.Western blot and immunohistochemistry methods were used to detect the expression changes of tight junction protein 1(ZO-1)and sealing protein Claudin-1 in kidney tissues.[Results]Four weeks of high-intensity exhaustive exercise induced exercise-induced proteinuria in rats,upregulated renal function markers serum Cre(P<0.01)and BUN(P<0.01),urine NGAL(P<0.01)and KIM-1(P<0.01)levels,and downregulated the expression of ZO-1(P<0.01)and Claudin-1(P<0.05)at the protein level in kidney tissues.[Conclusion]Four weeks of exhaustive exercise can cause sustained exercise-induced proteinuria in rats and lead to exercise-induced kidney injury in rats.Long-term exhaustive exercise may affect the integrity of renal tubular epithelial tight junction structure by down regulating renal tight junction proteins.

关 键 词：力竭运动 紧密连接蛋白 肾功能 

分 类 号：G804.7[文化科学—运动人体科学]