石斛酚抑制碱烧伤大鼠角膜新生血管的实验研究  

Experimental study of the inhibition of gigantol on corneal neovascularization in rats by alkali burn

作　　者：蒋波 田莎 李涛 李春霞 周晓东 JIANG Bo;TIAN Sha;LI Tao;LI Chunxia;ZHOU Xiaodong(Department of Ophthalmology,Jinshan Hospital Affiliated to Fudan University,Shanghai 201508,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Ophthalmology,Shanghai Hospital of Integrative Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200082,China)

机构地区：[1]复旦大学附属金山医院眼科,上海201508 [2]上海中医药大学,上海201203 [3]上海中医药大学附属上海市中西医结合医院眼科,上海200082

出　　处：《中国实验动物学报》2024年第7期846-855,共10页Acta Laboratorium Animalis Scientia Sinica

基　　金：上海市金山区医药卫生科技创新资金项目(2022-WS-14);上海市虹口区第二轮“国医强优”三年行动计划(2022-2024)(HKGYQYXM-2022-13)。

摘　　要：目的 探讨石斛酚抑制碱烧伤大鼠角膜新生血管(corneal neovascularization, CNV)的作用。方法 构建SD大鼠角膜碱烧伤模型,随机分成正常对照组、模型对照组、低浓度石斛酚组、高浓度石斛酚组、阿柏西普组,每组各10只。低浓度石斛酚组、高浓度石斛酚组、阿柏西普组分别于伤后第1天结膜下注射2.5 mg/0.05 mL、5 mg/0.05 mL石斛酚,2 mg/0.05 mL阿柏西普。伤后第3、7、14天,观察并计算角膜新生血管占全角膜面积的百分比、角膜混浊评分并测量角膜厚度。碱烧伤第14天,处死全部大鼠,通过苏木素-伊红(HE)染色和免疫组化观察角膜组织中VEGF和CD34蛋白表达水平,以及通过ELISA检测各组VEGF、IL-1β、TNF-α的蛋白含量。结果 碱烧伤后第7、14天,低浓度石斛酚组、高浓度石斛酚组、阿柏西普组角膜新生血管面积占全角膜面积百分比均显著低于模型对照组(所有P<0.05)。碱烧伤后第14天,高浓度石斛酚组角膜混浊评分显著小于模型对照组(P<0.05),模型对照组、低浓度石斛酚组角膜厚度均显著高于正常对照组(所有P<0.001)。但是,高浓度石斛酚组、阿柏西普组角膜厚度与正常对照组相比较,均无显著性差异(所有P>0.05)。此外,低浓度石斛酚组、高浓度石斛酚组、阿柏西普组角膜组织中VEGF、IL-1β、TNF-α蛋白表达均显著低于模型对照组(所有P<0.01)。结论 结膜下注射石斛酚对碱烧伤大鼠角膜新生血管有抑制作用,并能促进角膜水肿的吸收。Objective To investigate the inhibition of gigantol on corneal neovascularization(CNV)in rats after corneal alkali burn.Methods Animal models of corneal alkali burn were made with SD rats,which were divided into normal control group,model control group,low-concentration gigantol group,high-concentration gigantol group and aflibercept group,with 10 rats in each group.The rats in low-concentration gigantol group,high-concentration gigantol group and aflibercept group were treated with 2.5 mg/0.05 mL gigantol,5 mg/0.05 mL gigantol,and 2 mg/0.05 mL aflibercept by subconjunctival injection after modeling.The CNV,corneal opacity score,and thickness of the cornea were observed and compared on the 3rd,7th,and 14th days after alkali burn.The ratio of CNV area to corneal area was calculated.On the 14th day,all rats were sacrificed.Hematoxylin and eosin staining and immunohistochemistry were used to detect the expression of CD34 and VEGF.The protein expression of VEGF,IL-1β,and TNF-αwas detected by ELISA.Results On the 7th and 14th days after alkali burn,the percentages of CNV to total corneal area in low-concentration gigantol group,high-concentration gigantol group and aflibercept group were significantly smaller than those in model control group(all P<0.05).On the 14th day,the corneal opacity score was lower in high-concentration gigantol group than model control group(P<0.05).The corneal thickness in model control group and low-concentration gigantol group were significantly greater than that in normal control group(all P<0.001).However,the corneal thickness in highconcentration gigantol group and aflibercept group were not significantly different from that in normal control group(all P>0.05).In addition,the protein expression of VEGF,IL-1β,and TNF-αin corneal tissues in low-concentration gigantol group,high-concentration gigantol group and aflibercept group were significantly lower than that in model control group(all P<0.01).Conclusions Gigantol administration by subconjunctival injection can inhibit the formation

关 键 词：石斛酚 阿柏西普 碱烧伤 角膜新生血管 VEGF 

分 类 号：Q95-33[生物学—动物学]