兰香草对寒凝血瘀证原发性痛经大鼠的防治作用及机制研究  

Preventive and therapeutic effects and mechanisms of Caryopteris incana in a rat model of primary dysmenorrhea with cold coagulation and blood stasis syndrome

作　　者：陈美安 黄凤 梁晓庆 蓝小琪 崔祥祥 曾雪琪 CHEN Meian;HUANG Feng;LIANG Xiaoqing;LAN Xiaoqi;CUI Xiangxiang;ZENG Xueqi(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530001,China)

机构地区：[1]广西中医药大学药学院,南宁530001

出　　处：《中国实验动物学报》2024年第7期879-888,共10页Acta Laboratorium Animalis Scientia Sinica

基　　金：广西高校中青年教师科研基础能力提升项目(2022KY0296);广西中医药大学自然科学研究项目(2020MS004)。

摘　　要：目的探讨兰香草水煎液对寒凝血瘀证原发性痛经大鼠的防治效果及其作用机制。方法随机将60只健康SPF级SD雌性大鼠分为正常组、模型组、布洛芬组、兰香草高剂量组、兰香草中剂量组、兰香草低剂量组,每组10只。除正常组外,其余各组采用寒冷刺激联合苯甲酸雌二醇以及缩宫素建立寒凝血瘀证原发性痛经大鼠模型。于造模第5天开始灌胃给药,连续给药10 d。观察记录大鼠症状表现,测定大鼠扭体反应情况、血液流变学指标,测定大鼠血清中血浆血栓素B2(thromboxane B2,TXB_(2))、6-酮前列环素F_(1α)(6-Ketoprostacyclin F_(1α),6-Keto-PGF_(1α))、雌二醇(estradiol,E_(2))和孕酮(progesterone,PROG)水平,测定大鼠子宫组织中前列腺素F_(2α)(prostaglandin F_(2α),PGF_(2α))、前列腺素E_(2)(prostaglandin E_(2),PGE_(2))、一氧化氮(nitric oxide,NO)和钙离子(calcium,Ca^(2+))水平,以及计算子宫、卵巢脏器指数并观察其组织病理形态变化。结果与正常组比较,模型组大鼠出现明显寒凝血瘀证症状及扭体反应,子宫、卵巢组织形态出现明显增生、炎症、水肿等病变,血浆粘度、红细胞压积和全血粘度均明显升高(P<0.01),血清中TXB_(2)、E_(2)水平和E_(2)/PROG比值明显升高(P<0.01)以及6-Keto-PGF_(1α)和PROG水平明显降低(P<0.01),子宫指数和卵巢指数均明显增高(P<0.01),子宫组织中PGF_(2α)、Ca^(2+)水平和PGF_(2α)/PGE_(2)比值明显升高(P<0.01)以及PGE_(2)、NO水平明显降低(P<0.01)。与模型组比较,兰香草水煎液可明显改善模型大鼠的症状表现和子宫、卵巢组织形态病变,显著延长扭体反应潜伏时间和降低扭体次数(P<0.01),显著降低血浆黏度、红细胞压积和全血粘度(P<0.01),降低血清中TXB_(2)、E_(2)水平及E_(2)/PROG比值和升高血清中6-Keto-PGF_(1α)、PROG水平,降低子宫指数和卵巢指数(P<0.01,P<0.05),降低子宫组织中PGF_(2α)、Ca^(2+)水平及PGF_(2α)/PGE_(2)比值(P<0.01,Objective To explore the preventive and therapeutic effects of Caryopteris incana decoction in a rat model of primary dysmenorrhea with cold coagulation and blood stasis syndrome.Methods Sixty healthy specific pathogen-free SD female rats were randomly divided into six groups of ten rats each:normal group,model group,ibuprofen group,C.incana high-dose group,C.incana medium-dose group,and C.incana low-dose group.All groups except the normal group were treated with cold stimulation combined with estradiol benzoate and oxytocin to establish a rat model of primary dysmenorrhea with cold coagulation and blood stasis syndrome.On the fifth day of modeling,the rats were intragastrically administered the study drugs for 10 days.Their symptoms were observed and recorded.The writhing response and hemorheological indices were measured.The serum levels of TXB_(2),6-keto-prostaglandin F_(1α)(6-Keto-PGF_(1α)),estradiol(E_(2)),and progesterone(PROG)were measured.Additionally,the levels of prostaglandin F_(2α)(PGF_(2α)),prostaglandin E_(2)(PGE_(2)),nitric oxide(NO),and calcium(Ca^(2+))in the uterine tissues were measured.The organ indices of the uterus and ovary were calculated,and histopathological changes were observed.Results Compared with the normal group,the rats in the model group showed obvious symptoms of cold coagulation and blood stasis syndrome and writhing reaction.The morphology of uterus and ovary showed obvious hyperplasia,inflammation,edema and other lesions.The plasma viscosity,packed cell volume and whole blood viscosity were significantly increased(P<0.01).The serum levels of thromboxane B_2 and E_(2)and the E_(2)/PROG ratio were significantly increased(P<0.01),the levels of 6-Keto-PGF_(1α)and PROG were significantly decreased(P<0.01).The uterine index and ovarian index were significantly increased(P<0.01).The levels of PGF_(2α)and Ca^(2+)and the PGF_(2α)/PGE_(2)ratio in uterine tissue were significantly increased(P<0.01),while the levels of PGE_(2)and NO were significantly decreased(P<0.01).Compared wit

关 键 词：兰香草 寒凝血瘀证 原发性痛经 大鼠 

分 类 号：Q95-33[生物学—动物学]