龙岩山麻鸭胚胎肝间充质干细胞的分离、培养和鉴定  

Isolation,Culture and Identification of Liver Mesenchymal StemCells in Longyan ShanMa Duck

作　　者：张月 吴琼 王蕾 向仕玺 林明昕 吴凯琪 林睿愉 ZHANG Yue;WUQiong;WANG Lei;XIANG Shixi;LINMingxin;WUKaiqi;LINRuiyu(College of Life Sciences,Longyan University,Longyan 364012,China;Fujian Agricultural and Forestry University,Fuzhou 350002,China)

机构地区：[1]龙岩学院生命科学学院,福建龙岩364012 [2]福建农林大学,福建福州350002

出　　处：《特产研究》2024年第4期8-11,共4页Special Wild Economic Animal and Plant Research

基　　金：福建省科技厅引导性项目(2020N0034)。

摘　　要：为探索龙岩山麻鸭体外细胞保存方式,本研究建立了胚胎肝间充质干细胞的体外培养体系,从而达到利用细胞保护优异物种资源的目的。选用18~20日龄健康龙岩山麻鸭鸭胚通过胰蛋白酶消化分离出肝间充质干细胞(LMSCs),进行体外培养,细胞形态观察。通过免疫荧光检测CD29和CD44表面标记物,并进行阳性率分析。龙岩山麻鸭胚胎LMSCs体外增殖能力良好。LMSCs阳性表达了CD29和CD44,阳性表达率分别为95.20%和93.46%。体外分离培养出龙岩山麻鸭胚胎LMSCs,可为水禽干细胞研究提供研究思路,并为龙岩山麻鸭种质资源保护和利用提供参考。In order to explore the in vitro cell preservation method of Longyan Shanma duck,this study established an in vitro culture system of embryonic liver mesenchymal stem cells,so as to achieve the purpose of using the cells to protect the excellent species resources.Liver mesenchymal stemcells(LMSCs)were isolated by trypsin digestion from18~20 days old healthy Longyan Shanma duck embryos and cultured in vitro for cell morphology observation.CD29 and CD44 surface markers were detected by immunofluorescence,and the positive rate was analyzed.LMSCs from Longyan Shanma duck embryos proliferated well in vitro,and LMSCs positively expressed CD29 and CD44,with positive expression rates of 95.20%and 93.46%,respectively.LMSCs were isolated and cultured in vitro fromembryonic liver of Longyan Shanma duck.It can provide research ideas for waterfowl stem cell research,and provide reference for the conservation and utilization of germplasm resources of Longyan Shanma duck.

关 键 词：龙岩山麻鸭 肝脏 间充质干细胞 免疫荧光 

分 类 号：S834[农业科学—畜牧学]