前列桂芍颗粒对丙酸睾酮诱导的良性前列腺增生大鼠的影响  

The Effect of Qianlie Guishao Granule on Benign Prostatic Hyperplasia Rats Induced by Testosterone Propionate

作　　者：李会影[1] 杨振[1] 张红岩[1] 于映霞 宋莲莲[1] LI Huiying;YANG Zhen;ZHANG Hongyan;YUYingxia;SONG lianlian(Jilin Academy of Traditional Chinese Medicine,Changchun 130012,China)

机构地区：[1]吉林省中医药科学院,吉林长春130012

出　　处：《特产研究》2024年第4期82-85,共4页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划项目(20220204036YY)。

摘　　要：探讨前列桂芍颗粒对丙酸睾酮诱导的良性前列腺增生大鼠模型的影响。选择SPF级健康8周龄雄性SD大鼠60只,体质量280~300 g。按体重随机分为6组,空白对照组、模型对照组、前列桂芍颗粒高剂量组、前列桂芍颗粒中剂量组、前列桂芍颗粒低剂量组和阳性对照组,每组10只。灌胃给药4周后,取血,制备血清,测定血清睾酮、雌二醇含量;完整摘取大鼠前列腺组织,测湿重,计算前列腺指数(PI);将前列腺组织进行固定、HE染色,观察病理组织学变化。与模型对照组比较,前列桂芍颗粒高剂量组的血清睾酮显著降低(P<0.001),雌二醇含量明显降低(P<0.01);前列桂芍颗粒中剂量组的血清睾酮、雌二醇含量均有所降低(P<0.05);前列桂芍颗粒高剂量组的前列腺组织湿重明显降低(P<0.01),PI略降低(P<0.05);前列桂芍颗粒低剂量组的前列腺组织湿重有所降低(P<0.05);阳性对照组的PI略降低(P<0.05)。病理组织学检查发现,前列桂芍颗粒高剂量组效果较好,可以明显缓解前列腺间质水肿和充血,减轻前列腺间质炎细胞浸润状况,减少前列腺炎的发生。前列桂芍颗粒对丙酸睾酮诱导的良性前列腺增生大鼠具有明显的抑制作用,可以调节机体的性激素水平变化,值得进一步开发利用。To investigate the effect of Qianlie guishao granule on testosterone propionate-induced Benign prostatic hyperplasia rat model.60 healthy 8-week-old male SD rats with body weight of 280~300g were selected.They were randomly divided into 6 groups according to body weight:blank control group,model control group,high dose Qianlie Guishao granule group,middle dose Qianlie Guishao granule group,low dose Qianlie Guishao granule group and positive control group,10 in each group.After 4 weeks of oral administration,blood samples were collected to prepare serum,and the contents of testosterone and estradiol in serumwere measured,and the prostate tissue was collected to measure the wet weight and calculate the prostate index(PI).The prostatic tissues were fixed and stained by HE,and the histopathological changes were observed.Compared with the model control group,the serumtestosterone and estradiol content in the high dose group were significantly decreased(P<0.001),and the serum estradiol content was significantly decreased(P<0.01).The contents of serum testosterone and estradiol in the middle dose group were lower than those in the high dose group(P<0.05),and the wet weight and PI of prostate tissue in the high dose group were lower than those in the control group(P<0.01,P<0.05).The wet weight of prostate tissue in the low-dose group was lower than that in the positive control group(P<0.05),and the Pi in the positive control group was lower than that in the low-dose group(P<0.05).Histopathological examination showed that the high-dose group of Qianlie Guishao granule had a better effect,which could obviously relieve the edema and congestion of the prostatic stroma,and alleviate the infiltration of inflammatory cells in the prostatic stroma,reduce the occurrence of prostatitis Qianlie guishao granule has an obvious inhibitory effect on testosterone propionate-induced benign prostatic hyperplasia rats,and can regulate the changes of sex hormone levels in the body,it is worthy of further development and utilization.

关 键 词：前列桂芍颗粒 丙酸睾酮 前列腺增生 

分 类 号：R285.5[医药卫生—中药学]