生长素在多倍体蒲公英组培中的应用研究  

Application of Auxin Analogues in Tissue Culture of Polyploid Dandelion

作　　者：马千一 王洁 张琦琦 李金凤 张建 王宇[1] MA Qianyi;WANG Jie;ZHANG Qiqi;LI Jinfeng;ZHANG Jian;WANG Yu(Faculty of Agriculture,Forestry and Food Engineering,Yibin University,Yibin 644000,China)

机构地区：[1]宜宾学院农林与食品工程学部,四川宜宾644000

出　　处：《特产研究》2024年第4期86-90,共5页Special Wild Economic Animal and Plant Research

基　　金：宜宾学院高层次人才“启航”计划项目(2021QH014);宜宾学院大学生创新训练计划项目(S202210641099)。

摘　　要：本文旨在研究不同生长素种类和浓度在蒲公英组培中的效果,为多倍体蒲公英的组培快速繁殖提供参考。本研究以秋水仙素诱导多倍体的药用蒲公英叶片为材料,采用组织培养方法,研究不同生长素种类和浓度在多倍体蒲公英组培中的效果。结果表明,诱导愈伤组织的最佳培养基为MS+2,4-D(0.1 mg/L)+NAA(0.5 mg/L)+6-BA(1.5 mg/L),接种6 d出现愈伤,诱导率为100%,愈伤组织湿润致密,绿色有光泽,自发产生大量不定芽,平均芽数为10.66个;最佳增殖分化培养基为:MS+NAA(0.5 mg/L)+6-BA(1.5 mg/L)+2,4-D(0.1 mg/L),其分化率达到97.74%,增殖倍数为10.8;最佳生根培养基为:1/2MS+IBA(1.5 mg/L)+NAA(1 mg/L)+活性炭(0.8 g/L),接种8~9 d即可生根,根系粗壮,生根数量为11.34,生根频率最高可达93.47%;炼苗移栽植株成活率达到100%。不同生长素种类和浓度对蒲公英组培效果有明显差异,2,4-D浓度为0.1mg/L是蒲公英叶片愈伤组织诱导的最佳浓度。本研究为多倍体蒲公英组织培养体系的建立提供了技术支撑,并对多倍体蒲公英组织培养进一步深化研究奠定基础。The aimof this paper is to study the effects of different auxin species and concentrations on tissue culture of dandelion,and provide reference for the rapid utilization of polyploid dandelion.In this study,the effects of different auxin types and concentrations on polyploid dandelion tissue culture were studied by using colchicin-induced doubled medicinal dandelion leaves as materials.The results showed that the optimal medium for callus induction was MS+2,4-D(0.1 mg/L)+NAA(0.5 mg/L)+6-BA(1.5 mg/L).Callus induction rate was 100%on 6 days after inoculation.Callus were moist and dense,green and shiny,and a large number of advective buds were spontaneously generated,with an average number of 10.66 buds.The optimal mediumfor proliferation and differentiation wasMS+NAA(0.5 mg/L)+6-BA(1.5 mg/L)+2,4-D(0.1 mg/L),the differentiation rate reached 97.74%and the multiplication ratio was 10.8.The optimal rooting medium was 1/2MS+IBA(1.5 mg/L)+NAA(1mg/L)+activated carbon(0.8 g/L),and the roots were robust,the number of roots was 11.34,and the rooting frequency was up to 93.47%.The survival rate of transplanting plants reached 100%.The effects of different auxin and auxin concentration on tissue culture of dandelion are obviously different.The concentration of auxin should be adjusted in time according to the variety and absorption characteristics of experimental materials to improve the survival rate and utilization rate of dandelion.This study provides experimental support for the establishment of polyploid dandelion tissue culture systemand lays the foundation for further deepening research.

关 键 词：药用蒲公英 组织培养 生长素 增殖分化 褐化 

分 类 号：S649[农业科学—蔬菜学]