红大戟醇提物灌胃对小鼠支气管哮喘的治疗作用及其机制  

Therapeutic effect and mechanism of ethanol extract of Knoxia roxburghii on mice with bronchial asthma

作　　者：李绍花 陈福长 袁圆 龙玉淮 邱斌 何红平 黄丰 李宝晶 LI Shaohua;CHEN Fuchang;YUAN Yuan;LONG Yuhuai;QIU Bin;HE Hongping;HUANG Feng;LI Baojing(College of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China;不详)

机构地区：[1]云南中医药大学中药学院,昆明650500 [2]云南中医药大学云南省南药可持续利用研究重点实验室 [3]云南省教育厅中药防治哮喘重点实验室

出　　处：《山东医药》2024年第23期16-20,共5页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(82260832,81860760,82060751);云南省中医联合专项(202101AZ070001-159);云南省南药可持续利用研究重点实验室(202105AG070012);云南省科技厅基础研究重点项目(2019FA035)。

摘　　要：目的观察红大戟醇提物灌胃对支气管哮喘(简称哮喘)小鼠呼吸功能的改善作用,并探讨其可能的机制。方法将32只Balb/c雌性小鼠适应性喂养1周,随机分为正常对照组、模型组、地塞米松组、红大戟醇提物组,每组8只。模型组、地塞米松组、红大戟醇提物组以腹腔注射及雾化卵蛋白(OVA)激发液的方式建立哮喘模型,红大戟醇提物组、地塞米松组分别给予红大戟醇提物200 mg/kg、地塞米松1 mg/kg灌胃,正常对照组和模型组均给予等量生理盐水灌胃。各组末次雾化激发第2天,使用全身体积描记系统检测0、6.25、12.5、25、50、100 mg/mL乙酰甲胆碱(Mch)作用后的气道反应性指标呼气间歇(Penh)值,采用ELISA法检测血清总免疫球蛋白E(IgE)、卵清蛋白特异性IgE抗体(OVA-IgE)和支气管肺泡灌洗液(BALF)中的Th1细胞因子γ-干扰素(IFN-γ)、Treg细胞因子白细胞介素10(IL-10)以及Th2细胞因子(IL-4、IL-5、IL-9、IL-13)。各组小鼠处死后取右肺中叶组织,HE及PAS染色后观察肺组织病理情况。结果与正常对照组同浓度Mch作用后比较,模型组小鼠25、50、100 mg/mL Mch作用后的Penh值均升高(P均<0.05)。与模型组同浓度Mch作用后比较,地塞米松组、红大戟醇提物组小鼠50、100 mg/mL Mch作用后的Penh值均降低(P均<0.05)。与正常对照组比较,模型组小鼠血清总IgE、OVA-IgE水平均升高,BALF中IL-10、IFN-γ水平均降低而IL-4、IL-5、IL-9、IL-13水平均升高(P均<0.05)。与模型组比较,地塞米松组、红大戟醇提物组小鼠血清总IgE、OVA-IgE水平均降低,BALF中IL-10、IFN-γ水平均升高而IL-4、IL-5、IL-9、IL-13水平均降低(P均<0.05)。与正常对照组比较,模型组小鼠肺组织出现气道炎症细胞浸润、杯状细胞增生和黏液高分泌等病理改变;与模型组比较,地塞米松组、红大戟醇提物组小鼠肺组织上述病理改变明显减轻。结论红大戟醇提物灌胃有助于降低哮喘小鼠Objective To observe the improvement effect of intragastric administration of ethanol extract of Knoxia roxburghii on respiratory function in bronchial asthma mice and to investigate its possible mechanism.Methods Thirtytwo Balb/c female mice were adaptively fed for 1 week and randomly divided into the control group,model group,dexa⁃methasone group and the ethanol extract of Knoxia roxburghii group,with 8 mice in each group.OVA sensitizing solution[OVA 1 mg/mL and Al(OH)35 mg/mL]was intraperitoneally injected into mice to establish the asthma models in the model group,dexamethasone group and the ethanol extract of Knoxia roxburghii group;mice in the ethanol extract of Knox⁃ia roxburghii group and the dexamethasone group were given intragastric administration of 200 mg/kg of ethanol extract of Knoxia roxburghii and 1 mg/kg of dexamethasone,respectively;mice in the normal control group and the model group were both given the same volume of normal saline.On the second day of the last nebulization stimulation in each group,the average enhanced pause(Penh)values after 0,6.25,12.5,25,50,and 100 mg/mL methachcholine(Mch)were measured using a whole-body plethysmography system.ELISA was used to detect serum total immunoglobulin E(IgE),ov⁃albumin specific-IgE(OVA-IgE),and Th1 type inflammatory factor gamma interferon(IFN-γ),Treg inflammatory inter⁃leukin-10(IL-10),and Th2 type inflammatory factors(IL-4,IL-5,IL-9,IL-13)in bronchoalveolar lavage fluid(BALF).After execution,we taken the middle lobe tissues of the right lung and observed the pathological changes of the lung tissues after HE and PAS staining.Results Compared with the control group,the Penh values of the model group mice increased after 25,50,and 100 mg/mL Mch treatment(all P<0.05).Compared with the model group,the Penh val⁃ues of the dexamethasone group and the ethanol extract of Knoxia roxburghii group decreased after 50 and 100 mg/mL Mch treatment(all P<0.05).Compared with the control group,the serum IgE and OVA-IgE levels of the model group in⁃c

关 键 词：红大戟醇提物 哮喘 气道反应性 炎症反应 Th1/Th2免疫平衡 

分 类 号：R563.9[医药卫生—呼吸系统]