溴氰菊酯不同暴露时间对小鼠神经行为的毒性作用研究  

Study on the neurotoxic effects of different exposure durations of deltamethrin on mice’s neurobehavioral performance

作　　者：马佩璇 吴敏嘉 陈阳 潘雯 邓雅琴 陈维奇 黄晓薇[1] MA Peixuan;WU Minjia;CHEN Yang;PAN Wen;DENG Yaqin;CHEN Weiqi;HUANG Xiaowei(Department of Toxicology,School of Public Health,Guangxi Key Laboratory of Environment and Health Research,Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学公共卫生学院毒理学系、广西环境与健康研究重点实验室、广西高校高发疾病预防与控制研究重点实验室,南宁530021

出　　处：《广西医科大学学报》2024年第7期982-988,共7页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.21966010);广西医科大学高水平创新团队及杏湖学者计划资助项目。

摘　　要：目的:探究溴氰菊酯(DM)不同暴露时间对小鼠神经行为的毒性作用。方法:将32只成年雄性C57BL/6J小鼠随机分为30 d(对照组、DM组)和90 d(对照组、DM组)4组,每组8只。DM组经口灌胃4.5 mg/kg(1/20 LD_(50))30 d或90 d,对照组给予等容量玉米油。采用Morris水迷宫(MWM)检测小鼠的学习记忆能力;苏木精—伊红(HE)染色及尼氏染色观察小鼠皮层组织病理学变化;检测皮层组织还原型谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)的含量。结果:MWM实验结果,30 d的DM组与对照组相比无显著性差异(P<0.05)。90 d的DM组小鼠在目标象限时间、目标象限路程和穿越平台次数均显著少于对照组(P<0.01)。大脑皮层组织GSH的含量,DM 90 d组低于对照组(P<0.05);SOD活力变化,DM 30 d组低于对照组(P<0.01);CAT活力变化,DM 90 d组低于对照组(P<0.01)。结论:低剂量DM不同暴露时间对成年雄性小鼠的学习记忆能力及抗氧化能力具有不良影响,且这种影响随暴露时间的延长而加剧,应重视DM长期低剂量暴露对神经行为的影响。Objective:To investigate the neurotoxic effects of deltamethrin(DM)on the neurobehavioral performance of mice subjected to different exposure durations.Methods:Thirty-two adult male C57BL/6J mice were randomly assigned into four groups,with eight animals in each group:30-day control,30-day DM,90-day control,and 90-day DM groups.The DM groups received oral gavage of 4.5 mg/kg DM(1/20 of the LD_(50))for 30 days or 90 days,while the control groups were administered an equivalent volume of corn oil.Learning and memory capabilities of mice were assessed using the Morris water maze(MWM).Cortical histopathological changes of the mouse cortex were observed through hematoxylin and eosin(HE)staining and Nissl staining.The levels of glutathione(GSH),superoxide dismutase(SOD),catalase(CAT),and malondialdehyde(MDA)in the cortical tissue were measured.Results:In the MWM test,no significant differences were observed between the 30-day DM group and the control group(P<0.05).However,the 90-day DM group showed significant decreases in the time spent in the target quadrant,the distance traveled in the target quadrant,and the number of platform crossings compared to the control group(P<0.01).The content of GSH in the cortical tissue of the 90-day DM group was lower than that of the control group(P<0.05).The activity of SOD in the 30-day DM group was lower than that of the control group(P<0.01),the 90-day DM group was lower than that of the control group(P<0.01),and the activity of CAT in the 90-day DM group was also lower than that of the control group(P<0.01).Conclusion:Different exposure durations of low doses of DM have adverse effects on the learning and memory capabilities and antioxidant capacity of adult male mice,with these effects intensifying over extended exposure durations.The potential impact of long-term,low-dose exposure to DM on neurobehavior should be taken into consideration.

关 键 词：溴氰菊酯 学习记忆能力 神经退行性病变 氧化应激 亚慢性毒性 

分 类 号：R994.3[医药卫生—毒理学]