姜黄素调控MAPK/NF-κB/AP-1信号通路抑制狼疮性肾炎小鼠炎性反应作用分析  

Analysis of Curcumin Regulating MAPK/NF-κB/AP-1 Signaling Pathway and Inhibiting Inflammatory Response in Lupus Nephritis Mice

作　　者：屈红丽 刘曼丽 李响 QU Hongi;LIU Mani;LI Zhong(Zhoukou Hospital of Traditional Chinese Medicine,Zhoukou 466000,Henan,China)

机构地区：[1]周口市中医院,河南周口466000

出　　处：《实用中医内科杂志》2024年第8期93-95,共3页Journal of Practical Traditional Chinese Internal Medicine

基　　金：河南省医学科技攻关计划项目(LHGJ20211014)。

摘　　要：目的 探讨姜黄素调控丝裂原活化蛋白激酶(MAPK)/核因子-κB(NF-κB)/激活蛋白-1(AP-1)信号通路抑制狼疮性肾炎(lupus nephritis, LN)小鼠炎性反应作用。方法 LN小鼠16只分为LN组和实验组,各8只;C57BL/6小鼠8只作为对照组。实验组小鼠给予200 mg/kg姜黄素灌胃,1次/d;LN组和对照组小鼠灌胃等体积氯化钠溶液,1次/d;各组小鼠连续灌胃4周。运用全自动生化分析仪测定Scr和BUN水平;运用BCA蛋白测定试剂盒说明书测定尿蛋白水平;运用ELISA法测定白细胞介素1β(IL-1β)、血清白细胞介素6(IL-6)和肿瘤坏死因子-a (TNF-a)水平;运用Western Blot法测定MAPK/NF-κB/AP-1蛋白表达。结果 LN组和实验组Scr和BUN水平高于对照组(P<0.05);实验组Scr和BUN水平低于LN组(P<0.05)。LN组和实验组尿蛋白水平高于对照组(P<0.05);实验组尿蛋白水平低于LN组(P<0.05)。LN组和实验组血清IL-1β、IL-6和TNF-α水平高于对照组(P<0.05);实验组血清IL-1β、IL-6和TNF-α水平低于LN组(P<0.05)。LN组和实验组MAPK蛋白灰度值、NF-κB蛋白灰度值和AP-1蛋白灰度值高于对照组(P<0.05);实验组MAPK蛋白灰度值、NF-κB蛋白灰度值和AP-1蛋白灰度值低于LN组(P<0.05)。结论 姜黄素可通过下调MAPK/NF-κB/AP-1蛋白表达而抑制LN小鼠炎性反应,且可改善肾功能。Objective To investigate the inhibitory effect of curcumin on the inflammatory response in lupus nephritis mice by regulating the mitogen activated protein kinase(MAPK)/nuclear factor kappa B(NF-κB)/activator protein-1(AP-1) signaling pathway. Methods 16 LN mice were divided into LN group and experimental group, each 8 mice;Eight C57BL/6 mice were used as the control group. The experimental group mice were given 200 mg/kg curcumin by gavage, once a day;LN group and control group mice were given equal volume of physiological saline by gavage, once a day;Each group of mice was orally administered continuously for 4 weeks. Using a fully automated biochemical analyzer to measure the levels of Scr and BUN;Use the instructions of the BCA protein assay kit to measure urinary protein levels;Using ELISA method to measure the levels of IL-1 β, IL-6, and TNF-α;Using Western Blot method to determine the expression of MAPK/NF-κB/AP-1 protein. Results The LN group and experimental group levels of Scr and BUN higher control group(P<0.05);experimental group levels of Scr and BUN lower LN group(P<0.05). The LN group and experimental group urinary protein levels higher control group(P<0.05);experimental group urine protein level lower LN group(P<0.05). The LN group and experimental group serum levels of IL-1 β, IL-6, and TNF-α higher control group(P<0.05);experimental group serum levels of IL-1 β, IL-6, and TNF-α lower LN group(P<0.05). The LN group and experimental group gray values of MAPK protein, NF-κB protein, and AP-1 protein higher control group(P<0.05);experimental group gray values of MAPK protein, NF-κB protein, and AP-1 protein lower LN group(P<0.05). Conclusion Curcumin can inhibit the inflammatory response of LN mice and improve renal function by downregulating the expression of MAPK/NF-κ B/AP-1 protein.

关 键 词：姜黄素 丝裂原活化蛋白激酶 核因子-ΚB 激活蛋白-1 狼疮性肾炎 炎性反应 

分 类 号：R285.5[医药卫生—中药学]