秦岭箭叶淫羊藿对骨质疏松大鼠骨代谢及胫骨骨微结构的影响  

Experimental study on the effects of epimedium sagittatum on bone metabolism and tibial bone microstructure in osteoporosis rats

作　　者：李永志 韩礼军 李智斌 郭东 李伟 张博栋 Li Yongzhi;Han Lijun;Li Zhibin;Guo Dong;Li Wei;Zhang Bodong(Department of Orthopedics and Traumatology,Shangluo Traditional Chinese Medicine Hospital,Shaanxi Province,Shangluo 726000,China)

机构地区：[1]陕西省商洛市中医医院骨伤二科,726000

出　　处：《疑难病杂志》2024年第8期993-998,1001,共7页Chinese Journal of Difficult and Complicated Cases

基　　金：陕西省中医药管理局课题项目(2021-GJ-JC015)。

摘　　要：目的探究秦岭箭叶淫羊藿(EPI)对骨质疏松(OP)大鼠骨代谢及胫骨骨微结构的影响。方法2023年8月—2024年1月于西安迪赛生物医药有限责任公司实验室进行实验,将50只大鼠随机分为Sham组、Model组、EPI低剂量(EPI-L)组、EPI高剂量(EPI-H)组、EPI-H+YC-1[缺氧诱导因子-1α(HIF-1α)抑制剂]组;除Sham组外,其余组采用双侧卵巢切除术构建OP大鼠模型。ELISA检测大鼠血清白介素10(IL-10)、IL-6、肿瘤坏死因子α(TNF-α)、血钙(Ca)、血磷(P)、骨保护素(OPG)、骨钙素(OCN)、碱性磷酸酶(ALP);双能X射线骨密度仪检测大鼠胫骨密度(BMD);Micro-CT检测胫骨骨微结构;HE染色观察胫骨组织形态;Western blot检测骨形成蛋白(BMP-2)、Runt相关转录因子2(Runx2)、半胱氨酸蛋白酶-3(cleaved caspase-3)、HIF-1α、血管内皮生长因子(VEGF)、血管内皮生长因子受体2(VEGFR-2)蛋白水平。结果与Sham组比较,Model组大鼠胫骨骨微结构和骨组织形态明显受损,血清IL-6、TNF-α、ALP水平、胫骨骨表面积与体积比、骨小梁分离度和胫骨骨组织cleaved caspase-3蛋白表达水平显著升高(P均<0.05),血清IL-10、OPG、OCN、Ca和P水平、胫骨BMD、骨小梁厚度、胫骨骨组织BMP-2、Runx2、HIF-1α、VEGF、VEGFR-2蛋白表达水平显著降低(P均<0.05);与Model组比较,EPI-L组和EPI-H组大鼠胫骨骨微结构和骨组织形态受损明显减轻,血清IL-6、TNF-α、ALP水平、胫骨骨表面积与体积比、骨小梁分离度、胫骨骨组织cleaved caspase-3蛋白表达水平显著降低(P均<0.05),血清IL-10、OPG、OCN、Ca和P水平、胫骨BMD、骨小梁厚度、胫骨骨组织BMP-2、Runx2、HIF-1α、VEGF、VEGFR-2蛋白表达水平显著升高(P均<0.05);与EPI-H组比较,EPI-H+YC-1组上述指标均被部分逆转(P均<0.05)。结论EPI可降低OP大鼠炎性反应,调节骨代谢平衡,改善胫骨骨微结构,可能与激活HIF-1α/VEGF/VEGFR-2信号通路有关。Objective To investigate the effects of epimedium sagittatum(EPI)on bone metabolism and tibial bone microstructure in osteoporosis(OP)rats.Methods Rats were randomly separated into Sham group,Model group,EPI low-dose(EPI-L)group,EPI high-dose(EPI-H)group,and EPI-H+YC-1(HIF-1αinhibitor)group.Bilateral oophorectomy was performed to construct an OP rat model.ELISA was applied to detect serum IL-10,IL-6,TNF-α,serum calcium(Ca),serum phosphorus(P),osteoprotegerin(OPG),osteocalcin(OCN),and alkaline phosphatase(ALP)in rats.Dual energy X-ray bone density instrument was applied to detect tibial bone density(BMD)in rats.Micro CT was applied to detect the microstructure of tibia bone.HE staining was applied to observe the morphology of tibial tissue.Bone morphogenetic protein(BMP-2),RUNt-associated transcription factor 2(Runx2),and cysteine proteinase-3(cleaved)were detected by Western blot Protein levels of caspase-3,hypoxia inducible factor HIF-1α(HIF-1α),vascular endothelial growth factor(VEGF),and vascular endothelial growth factor receptor 2(VEGFR-2).Results Compared with the Sham group,the microstructure and tissue morphology of the tibia of rats in the Model group were obviously damaged,the ratio of tibial bone surface area to volume,trabecular separation,serum IL-6,TNF-α,ALP levels,and tibial bone tissue cleaved caspase-3 protein expression level were obviously increased(all P<0.05),the bone trabecular thickness,serum IL-10,OPG,OCN,S-Ca and S-P levels,tibial BMD,tibial bone tissue BMP-2,Runx2,HIF-1α,VEGF,and VEGFR-2 protein expression levels were obviously reduced(all P<0.05).Compared with the Model group,the damage to the microstructure and tissue morphology of the tibia of rats in the EPI-L and EPI-H groups was obviously reduced,the ratio of tibial bone surface area to volume,trabecular separation,serum IL-6,TNF-α,ALP levels,and tibial bone tissue cleaved caspase-3 protein expression level were obviously reduced(all P<0.05),the bone trabecular thickness,serum IL-10,OPG,OCN,S-Ca and S-P levels,tibial BMD,tib

关 键 词：骨质疏松 箭叶淫羊藿 骨代谢 胫骨 骨微结构 大鼠 

分 类 号：R681[医药卫生—骨科学]