S1PR1在瑞芬太尼诱发切口痛大鼠痛觉过敏中的作用:与背根神经节KCNQ2/3钾通道的关系  

Role of S1PR1 in remifentanil-induced hyperalgesia in rats with incisional pain:relationship with KCNQ2/3 potassium channels in dorsal root ganglia

作　　者：殷玲[1] 宋振华 靳晓迪 李清[1] 于泳浩[1] 王春艳[1] Yin Ling;Song Zhenhua;Jin Xiaodi;Li Qing;Yu Yonghao;Wang Chunyan(Department of Anesthesiology,Tianjin Medical University General Hospital,Tianjin Research China,Institute of Anesthesiology,Tianjin 300052,China)

机构地区：[1]天津医科大学总医院麻醉科、天津市麻醉学研究所,天津300052

出　　处：《中华麻醉学杂志》2024年第7期821-825,共5页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金(81600962);天津市自然科学基金面上项目(18JCYBJC94400)。

摘　　要：目的评价鞘氨醇-1-磷酸受体1(S1PR1)在瑞芬太尼诱发切口痛大鼠痛觉过敏中的作用及其与背根神经节KCNQ2/3钾通道的关系。方法取尾静脉置管成功的雄性SD大鼠48只,2~3月龄,体质量260~280 g,采用随机数字表法分为6组(n=8):对照组(C组)、S1PR1抑制剂组(FTY720)组(F组)、瑞芬太尼组(R组)、瑞芬太尼+S1PR1抑制剂(FTY720)组(RF组)、瑞芬太尼+切口痛组(RI组)和瑞芬太尼+切口痛+S1PR1抑制剂(FTY720)组(RIF组)。C组静脉输注生理盐水0.1 ml·kg^(-1)·min^(-1)60 min;F组在生理盐水注射前10 min鞘内注射FTY7203 nmol,尾静脉输注生理盐水0.1 ml·kg^(-1)·min^(-1)60 min;R组尾静脉输注瑞芬太尼1.0μg·kg^(-1)·min^(-1)60 min;RF组静脉输注瑞芬太尼1.0μg·kg^(-1)·min^(-1)60 min前10 min鞘内注射FTY7203 nmol;RI组建立大鼠切口痛模型,尾静脉输注瑞芬太尼1.0μg·kg^(-1)·min^(-1)60 min;RIF组瑞芬太尼输注前10 min鞘内注射FTY7203 nmol,随后制备大鼠切口痛模型,同时尾静脉输注瑞芬太尼1.0μg·kg^(-1)·min^(-1)60 min。于输注瑞芬太尼或生理盐水前24 h(T0)、停止输注瑞芬太尼或生理盐水后2、6、24和48 h(T1-4)时测定机械缩足反应阈(MWT)和热缩足潜伏期(TWL)。最后一次测定痛阈后处死大鼠,取L4-6节段背根神经节组织,分别采用Western blot法和实时定量PCR法测定背根神经节S1PR1、KCNQ2和KCNQ3及其mRNA的表达。结果与C组比较,R组和RI组T1-4时MWT降低,TWL缩短,背根神经节S1PR1及其mRNA表达上调,KCNQ2和KCNQ3及其mRNA表达下调(P<0.05),F组各指标差异无统计学意义(P>0.05);与R组比较,RI组T1-4时MWT降低,TWL缩短,背根神经节S1PR1及其mRNA表达上调,KCNQ2和KCNQ3及其mRNA表达下调,RF组T1-4时MWT升高,TWL延长,背根神经节S1PR1表达下调,KCNQ2和KCNQ3表达上调(P<0.05);与RI组比较,RIF组T1-4时MWT升高,TWL延长,背根神经节S1PR1及其mRNA表达下调,KCNQ2和KCNQ3及其mRNA表达上调(P<0.05)。结论S1PR1参与了瑞芬太尼诱发切口Objective To evaluate the role of sphingosine-1-phospho-1 receptor 1(S1PR1)in remifentanil-induced hyperalgesia in rats with incisional pain and the relationship with KCNQ2/3 potassium channels in the dorsal root ganglia(DRG).Methods Forty-eight male Sprague-Dawley rats with successful caudal vein catheterization,aged 2-3 months,weighing 260-280 g,were divided into 6 groups(n=8 each)using a random number table method:control group(group C),S1PR1 inhibitor group(FTY720)group(group F),remifentanil group(group R),remifentanil+S1PR1 inhibitor(FTY720)group(group RF),remifentanil+incision pain group(group RI)and remifentanil+incision pain+S1PR1 inhibitor(FTY720)group(group RIF).In group C,normal saline 0.1 ml·kg^(-1)·min^(-1)was intravenously infused for 60 min.In group F,FTY7203 nmol was intrathecally injected at 10 min before normal saline injection,and 0.1 ml·kg^(-1)·min^(-1)normal saline was infused into the caudal vein for 60 min.Remifentanil 1.0μg·kg^(-1)·min^(-1)was infused for 60 min through the caudal vein in group R.In RF group,FTY720(3 nmol)was intrathecally injected,and 10 min later remifentanil 1.0μg·kg^(-1)·min^(-1)was infused via the caudal vein for 60 min.The incisional pain model was established,and remifentanil 1.0μg·kg^(-1)·min^(-1)was infused via the caudal vein for 60 min in RI group.In RIF group,FTY7203 nmol was intrathecally injected at 10 min before remifentanil infusion,then the incisional pain model was developed,and remifentanil 1.0μg·kg^(-1)·min^(-1)was infused via the caudal vein at the same time for 60 min.The mechanical paw withdraw threshold(MWT)and thermal paw withdraw latency(TWL)were measured at 24 h before remifentanil or normal saline infusion(T 0)and 2,6,24 and 48 h after remifentanil or normal saline infusion(T 1-4).The rats were sacrificed after the last measurement of pain threshold,and the L 4-6 segments of the DRG were taken for determination of the expression of S1PR1,KCNQ2 and KCNQ3 protein and mRNA in the DRG by Western blot and real-time polymerase chain re

关 键 词：鞘氨醇-1-磷酸受体 瑞芬太尼 痛觉过敏 神经节 脊 KCNQ钾通道 

分 类 号：R614[医药卫生—麻醉学]