电针对2型糖尿病大鼠骨骼肌GLUT4表达影响的研究  

Effect of Electroacupuncture on the Expression GLUT4 of Skeletal Muscle in Type-2 Diabetic Rats

作　　者：楼巍敏[1] 林坚[2] 李海龙 夏婉 黄雄昂[2] 刘晓林[2] 卢璇 刘彩霞[1] LOU Weimin;LIN Jian;LI Hailong(Nursing Department,Zhejiang Hospital,Hangzhou 310013,China;Rehabilitation Department,Zhejiang Hospital)

机构地区：[1]浙江医院护理部,杭州310013 [2]浙江医院康复医学科

出　　处：《浙江中医药大学学报》2024年第6期649-654,共6页Journal of Zhejiang Chinese Medical University

基　　金：浙江省中医药科研基金项目(2020ZB001、2021ZA002);浙江省自然科学基金项目(LY21H170001)。

摘　　要：[目的]观察电针对2型糖尿病大鼠骨骼肌葡萄糖转运蛋白4(glucose transporter 4,GLUT4)表达的影响,探讨电针调节血糖的机制。[方法]将24只2型糖尿病大鼠随机分为空白对照组、运动干预组及电针组,每组8只,运动干预组大鼠给予2周跑台运动干预;空白对照组大鼠不给予任何干预;电针组大鼠开始将电针置于大鼠双侧足三里穴,进行连续波穴位刺激2周。分别检测各组大鼠治疗前后空腹血糖水平、胰岛素抵抗指数及治疗后大鼠骨骼肌细胞GLUT4水平。[结果]与干预前比较,运动干预组和电针组空腹血糖水平显著下降(P<0.001,P=0.028),胰岛素抵抗指数显著下降(P<0.001,P<0.001)。干预后组间比较,三组大鼠空腹血糖水平及胰岛素抵抗指数差异均有统计学意义(P<0.001),GLUT4表达水平差异有统计学意义(P<0.001)。[结论]电针刺激能改善2型糖尿病大鼠的胰岛素敏感性,并降低其血糖水平,其中调节骨骼肌细胞GLUT4水平可能是其调节血糖的一个重要机制。[Objective]To observe the effect of electroacupuncture on the expression of glucose transporter 4(GLUT4)of skeletal muscle in rats with type-2 diabetes mellitus,and to elucidate the mechanism of electroacupuncture on blood glucose regulation.[Methods]Twentyfour rats with type-2 diabetes were randomly divided into blank control group,exercise intervention group and electroacupuncture group,with 8 rats in each group.Rats in blank control group did normal activities.After 1 week of adaptation,rats in exercise intervention group began to run on the treadmill for 2 weeks.The rats in electroacupuncture group were adapted for 1 week,and then the electroacupuncture was placed at the Zhusanli acupoint on both sides with continuous wave stimulation for 2 weeks.Fasting blood glucose and insulin resistance index(IRI)were detected before and after the treatment,and the GLUT4 level of rat muscle cells was detected after the treatment.[Results]Compared with before intervention,the fasting blood glucose level of rats with type-2 diabetes in exercise intervention group and electroacupuncture group reduced significantly after intervention(P<0.001,P=0.028),and the IRI was reduced significantly(P<0.001,P<0.001).The differences of fasting blood glucose level and IRI were significant among the three groups after intervention(P<0.001).The difference of level of GLUT4 was also significant among the three groups after intervention(P<0.001).[Conclusion]Electroacupuncture stimulation can significantly decrease the level of blood glucose and IRI,and regulate the level of GLUT4 in skeletal muscle,which may be an important mechanism of regulation.

关 键 词：2型糖尿病 电针 运动干预 血糖 胰岛素抵抗指数 GLUT4 

分 类 号：R331[医药卫生—人体生理学]