19个秀珍菇菌株种质鉴定及遗传多样性分析  

Germplasm identification and genetic diversity analysis of 19 strains of Pleurotus pulmonarius

作　　者：孙悦 郝刘斌 冯璠 王春霞 郑素月 SUN Yue;HAO Liubin;FENG Fan;WANG Chunxia;ZHENG Suyue(College of Landscape Architecture and Ecological Engineering,Hebei University of Engineering,Handan 056038,China)

机构地区：[1]河北工程大学园林与生态工程学院,河北邯郸056038

出　　处：《食药用菌》2024年第4期246-250,255,共6页Edible and Medicinal Mushrooms

基　　金：河北省农业科技成果转化资助项目(编号202360101010014);河北省现代农业产业技术体系食用菌创新团队建设专项(编号HBCT2023090207)。

摘　　要：采用拮抗试验、酯酶同工酶测定和ISSR分子标记技术从形态学、蛋白质和分子角度对19个生产中常用的秀珍菇菌株进行鉴定分析和遗传多样性评价。最终3种方法测定结果高度一致,19个秀珍菇菌株可划分为四类:第一类有9个菌株,分别为1、7、8、9、10、11、15、16、17号;第二类有8个菌株,分别为2、3、4、5、6、13、14、19号;第三类有1个菌株,为12号;第四类有1个菌株,为18号。Using antagonism test,esterase isoenzyme assay and ISSR molecular marker technique,the morphological,protein,and molecular characteristics of 19 strains of Pleurotus pulmonarius,which were commonly used in production,were identified and evaluated for genetic diversity.The result showed that the three methods were highly consistent,and the 19 strains could be divided into four groups.The first group had 9 strains,including No.1,7,8,9,10,11,15,16 and 17.The second group had 8 strains,including No.2,3,4,5,6,13,14 and 19.The third group and the fourth group each had 1 strain,which were No.12 and No.18 respectively.

关 键 词：秀珍菇 拮抗试验 酯酶同工酶测定 ISSR分子标记技术 

分 类 号：S646[农业科学—蔬菜学]