HBV RNA用于监测慢性乙型肝炎患者临床治愈的研究和应用  

Research and application of HBV RNA detection in monitoring the clinical cure of chronic hepatitis B patients

作　　者：李玛[1] 闫凌 席云[1] 李尚霞 朱冬林[1] Ma Li;Ling Yan;Yun Xi;Shangxia Li;Donglin Zhu(Department of Laboratory,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou Guangdong 510630,China;General ICU,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou Guangdong 510630,China)

机构地区：[1]中山大学附属第三医院检验科,广东广州510630 [2]中山大学附属第三医院综合ICU,广东广州510630

出　　处：《中华临床实验室管理电子杂志》2024年第2期70-74,共5页Chinese Journal of Clinical Laboratory Management(Electronic Edition）

基　　金：广东省重点领域研发计划项目(2019B020231002)。

摘　　要：目的探讨HBV RNA检测在监测慢性乙型肝炎(CHB)患者治疗中的作用。方法应用实时荧光定量PCR检测56例CHB患者血清中HBV DNA及HBV RNA载量,并用电化学发光法定量检测同一时期患者血清中的HBsAg。对56例CHB患者的HBV DNA和HBV RNA检测值进行Wilcoxon符号秩检验,再将这两种检测值与HBsAg定量值进行Spearman秩相关分析,分析其中的相关性。结果56例CHB患者血清,HBV RNA和HBsAg检测均为阳性,其中有7例HBV DNA检测为阴性。HBV DNA与HBV RNA检测值之间差异有统计学意义(P<0.05)。HBV DNA、HBV RNA载量分别与HBsAg定量进行分析,二者与HBsAg定量均无相关性(均P>0.05)。结论CHB患者的HBV RNA、HBsAg阳性检出率高于HBV DNA。HBsAg与HBV RNA的阳性检出率具有一致性。Objective To investigate the role of HBV RNA detection in the treatment of chronic hepatitis B(CHB)patients.Methods HBV DNA and HBV RNA in serum of 56 patients with CHB were detected by real-time fluorescent quantitative PCR,and HBV surface antigen in serum of the same period was quantitatively detected by electrochemiluminescence.The Wilcoxon sign rank test was conducted on the detection values of HBV RNA and HBV DNA in 56 patients.Then,Spearman correlation analysis was made between the two values and the quantitative value of HBsAg to analyze the correlation.Results The serum of 56 patients with clinically diagnosis of CHB was positive for HBV RNA and HBsAg were,and 7 of them were negative for HBV DNA.The difference between HBV RNA and HBV DNA detection values was statistically significant(P<0.05).The HBV RNA and HBV DNA load were analyzed separately with HBsAg quantitation,and there was no correlation between HBV and HBsAg(all P>0.05).Conclusions The positive detection rate of HBV RNA and HBsAg was higher than that of HBV DNA.The positive detection of HBsAg and HBV RNA is consistent.

关 键 词：乙肝病毒RNA 乙肝病毒DNA 乙肝表面抗原 慢性乙型肝炎 

分 类 号：R512.62[医药卫生—内科学]