PPARα激动剂橙皮油素对小鼠摄食的影响  

The influence of auraptene as an agonist of PPARαon food intake in mice

作　　者：郭莉霞 殷钟意 邓佳 GUO Lixia;YIN Zhongyi;DENG Jia(Environmental and Resources Institute,Chongqing Technology and Business University,Chongqing 400067,China;Key Laboratory of Natural Medicine Research of Chongqing Education Commission,Chongqing 400067,China)

机构地区：[1]重庆工商大学环境与资源学院,重庆400067 [2]天然药物研究重庆高校市级重点实验室,重庆400067

出　　处：《生物学杂志》2024年第4期30-36,共7页Journal of Biology

基　　金：重庆市自然科学基金面上项目(CSTB2022NSCQ-MSX1420)。

摘　　要：探讨橙皮油素(Auraptene,AUR)的摄入对正常小鼠摄食的影响及其作用机制。将正常小鼠分为3组,正常对照组、AUR(2μmol/kg)组、AUR(1μmol/kg)组,连续灌胃给予AUR 2周,检测小鼠的累积摄食,检测血清、肝脏和下丘脑中胰岛素样生长因子(IGFs)、胰岛素样生长因子结合蛋白(IGFBPs)、下丘脑中厌食因子阿片促黑色素原(POMC)、过氧化物酶体增殖物激活受体α(PPARα)、靶基因肉毒碱棕榈酰基转移酶1A(CPT1A)mRNA和蛋白水平的变化。结果发现:AUR能够浓度依赖性地促进小鼠摄食,抑制下丘脑厌食因子POMC的表达(P<0.01),对肝脏IGFBP-1的表达有显著的促进作用(P<0.01),在血清和下丘脑中IGF-1的含量显著减少(P<0.01),但并未影响肝脏IGF-1的表达(P>0.05),同时,AUR显著增加了CPT1A在肝脏中的表达(P<0.01)。综上,AUR可显著促进小鼠摄食,其机制主要为AUR激活了PPARα,促使肝脏IGFBP-1表达增加,从而结合了血清中游离的IGF-1,使进入下丘脑的IGF-1含量减少,抑制了厌食因子POMC的表达,促进了小鼠摄食。The aim of this study was to investigate the influence of auraptene(AUR)on food intake in mice and its mechanism.Mice were randomly divided to three groups including control group,2μmol/kg AUR group and 1μmol/kg AUR group.AUR was continuously given by oral gavage for 2 weeks to measure the cumulative food intake of the mice,the expression of insulin-like growth factors(IGFs)in serum,liver and hypothalamus,insulin-like growth factor-binding proteins(IGFBPs)in liver,proopiomelanocortin(POMC)in hypothalamus.The levels of mRNA and protein of carnitine palmitoyltransferase 1A(CPT1A)as a peroxisome proliferator-activated receptorα(PPARα)target gene were also tested.The results showed that AUR promoted food intake in mice in a concentration-dependent manner,inhibited the expression of POMC(P<0.01),significantly promoted the expression of IGFBP-1 in liver(P<0.01),and significantly reduced the content of IGF-1 in serum and hypothalamus(P<0.01),but did not effect on IGF-1 in liver(P>0.05).Meanwhile,AUR significantly increased the expression of CPT1A in liver(P<0.01).In summary,these findings suggested that AUR could significantly promote food intake in mice.The main mechanism was that AUR promoted food intake in mice because of increasing the expression of IGFBP-1 in liver by PPARαactivation,which bound the free IGF-1 in serum to decrease IGF-1 in hypothalamus and inhibit the expression of POMC.

关 键 词：橙皮油素 摄食 胰岛素样生长因子结合蛋白 过氧化物酶体增殖物激活受体Α 肝脏 大脑 

分 类 号：Q4[生物学—生理学] Q786