转录因子NF-κB对鲤疱疹病毒Ⅱ型基因启动子的表达调控研究  

Regulation of the gene promoter of[STHX]Cyprinid herpesvirus 2 by the transcription factor NF-κB

作　　者：谢雅晴 龙晨 吕利群[2] XIE Yaqing;LONG Chen;LYU Liqun(National Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学国家水生动物病原库,上海201306 [2]上海海洋大学农业农村部淡水水产种质资源与利用重点实验室,上海201306

出　　处：《生物学杂志》2024年第4期88-93,共6页Journal of Biology

基　　金：国家现代农业产业技术体系建设专项(CARS-45-19)。

摘　　要：为深入探究NF-κB信号通路对CyHV-2感染过程的影响,在CyHV-2感染的GiCF细胞中加入NF-κB激动剂,通过RT-qPCR和Western Blot分析在感染24、72、96 h后CyHV-2编码基因在转录和翻译水平的表达情况。结果显示,在加入NF-κB激动剂后的72、96 h,CyHV-2编码基因转录和翻译水平均明显高于对照组,提示NF-κB促进CyHV-2编码基因的转录及翻译。为进一步阐明NF-κB如何影响CyHV-2的转录和翻译,利用生物信息学软件预测CyHV-2部分编码基因的启动子序列中和NF-κB的结合位点,采用双荧光素酶报告基因实验分析NF-κB对启动子活性的影响。结果表明,NF-κB过表达提高了病毒基因启动子活性,提示NF-κB可以通过促进CyHV-2基因的启动子活性来增强病毒复制水平。研究结果有助深入探究CyHV-2基因的表达翻译机制,也为开发基于NF-κB信号通路靶点的新的抗病毒抑制剂提供理论支撑。In order to investigate the mechanism of NF-κB in enhancing CyHV-2 replication,transcriptional and translational levels of CyHV-2 genes at 24,72 and 96 h post CyHV-2 infection in the presence of NF-κB agonist were monitored by RT-qPCR and Western Blot assays.The results showed that the transcriptional levels and protein levels of CyHV-2 genes were increased at 72 and 96 h in the presence of NF-κB agonist.To further elucidate how NF-κB affects the transcription and translation of CyHV-2,the binding sites of the promoter region for NF-κB were predicted and dual luciferase reporter gene experiments were performed to study the regulatory role of NF-κB on viral gene expression.The experimental results showed that NF-κB overexpression increased CyHV-2 promoter activity,further suggesting that NF-κB promotes viral replication by activating the promoter of CyHV-2 genes.This study should provide novel strategies for anti-CyHV-2 drugs and enhance the understanding of CyHV-2 replication.

关 键 词：鲤疱疹病毒Ⅱ型 NF-ΚB 启动子 转录调控 双荧光素酶报告基因 

分 类 号：Q753[生物学—分子生物学] S941.41[农业科学—水产养殖]