改良DMEM培养基对人芽囊原虫的培养效果观察  

EFFECTS OF ENHANCED DULBECCO′S MODIFIED EAGLE MEDIUM ON BLASTOCYSTIS HOMINIS CULTURE

作　　者：朱名超[1] 朱娅[2] 原慧真 郭飞波 王俊[1] 舒玲 ZHU Ming-chao;ZHU Ya;YUAN Hui-zhen;GUO Fei-bo;WANG Jun;SHU Ling(Department of Clinical Laboratory,The First People's Hospital of Tianmen,Tianmen 431700,Hubei,China;Department of Neurology,The First People's Hospital of Tianmen,Tianmen 431700,Hubei,China;College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,Henan,China)

机构地区：[1]天门市第一人民医院检验科,湖北天门431700 [2]天门市第一人民医院神经内科,湖北天门431700 [3]河南农业大学动物医学院,郑州450046

出　　处：《寄生虫与医学昆虫学报》2024年第2期72-77,共6页Acta Parasitologica et Medica Entomologica Sinica

基　　金：中央高校基本科研业务费专项基金(No.2042020kfxg05)。

摘　　要：目的探讨改良DMEM培养基培养人芽囊原虫(Blastocystis hominis,B.h)的效果。方法选取了329例临床样本分别用改良DMEM培养法与普通DMEM培养法和碘液染色涂片法对B.h的检出率进行比较;普通DMEM培养法与改良DMEM培养法中B.h形态、最低检出限、生长曲线等进行比较。结果329例临床样本中改良DMEM培养B.h检出阳性率为5.17%(17/329),碘液染色涂片法阳性率1.52%(5/329),差异有统计学意义(P<0.01)。B.h在两种培养基中均以空泡型为主,但改良DMEM更易见到颗粒型和二分裂型,生长高峰期为第72 h,达高峰期后维持时间较普通DMEM培养长。结论改良DMEM培养法优于普通DMEM培养法,可用于人芽囊原虫的体外增殖培养。Objective To investigate the effect of enhanced Dulbecco′s modified Eagle′s medium(DMEM)on the cultivation of Blastocystis hominis.Methods A total of 329 clinical samples were selected to compare the growth of B.hominis using enhanced DMEM and the iodine solution direct smear method.The morphology,minimum detection limit and growth curve of B.hominis were compared between the conventional DMEM culture medium and the enhanced DMEM culture method.Results In 329 clinical specimens,the positive rate for B.hominis was 5.17%(17/329)in the enhanced DMEM culture and 1.52%(5/329)in the iodine solution stained smears;the difference was statistically significant(P<0.01).Conclusion The enhanced DMEM culture method can be used for the proliferation and culture of B.hominis in vitro.

关 键 词：人芽囊原虫 改良DMEM培养法 碘液染色 形态观察 

分 类 号：R382.9[医药卫生—医学寄生虫学]