UPLC法同时测定炎可宁片中13个成分含量  

Simultaneous determination of 13 components in Yankening tablets by UPLC

作　　者：董晓茜[1] 鄢长余 马进[1] DONG Xiao-qian;YAN Chang-yu;MA Jin(Liaoning Institute of Traditional Chinese MedicineThe Second Hospital Affiliated to Liaoning University of Traditional Chinese Medicine,Shenyang 110034,China;Dalian Inspection and Certification Technical Service Center,Dalian 116021,China)

机构地区：[1]辽宁省中医药研究院(辽宁中医药大学附属第二医院),沈阳110034 [2]大连市检验检测认证技术服务中心,大连116021

出　　处：《药物分析杂志》2024年第7期1195-1201,共7页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用超高效液相色谱法同时测定炎可宁片中黄柏碱、黄连碱、黄芩苷、巴马汀、小檗碱、汉黄芩苷、黄芩素、芦荟大黄素、大黄酸、汉黄芩素、大黄素、大黄酚、大黄素甲醚13个成分含量。方法:采用Agilent Eclipse Plus-C_(18)(100 mm×2.1 mm,1.8μm)色谱柱,以乙腈-0.1%磷酸水为流动相,梯度洗脱,流速0.3 mL·min^(-1),测定波长210、254 nm,柱温40℃。结果:盐酸黄柏碱、盐酸黄连碱、黄芩苷、盐酸巴马汀、盐酸小檗碱、汉黄芩苷、黄芩素、芦荟大黄素、大黄酸、汉黄芩素、大黄素、大黄酚、大黄素甲醚的的线性范围分别为0.97~48.63、0.95~47.52、0.86~43.15、0.86~43.19、0.89~44.37、1.00~49.84、1.02~51.01、0.97~48.31、0.99~49.50、1.04~51.80、1.00~50.04、1.00~49.80、1.01~50.64μg·mL-1;平均回收率(n=6)分别为95.2%、96.7%、98.3%、94.1%、95.9%、97.6%、99.2%、96.6%、95.5%、97.2%、97.0%、97.8%、98.7%,RSD均<2.0%。3批样品中上述13个成分的含量分别为1.367~1.488(以盐酸黄柏碱计)、0.378~0.412(以盐酸黄连碱计)、4.611~5.505、0.324~0.407(以盐酸巴马汀计)、3.665~3.878(以盐酸小檗碱计)、1.107~1.682、0.392~0.941、0.076~0.105、0.097~0.116、1.059~1.213、0.149~0.167、0.213~0.239、0.047~0.059 mg·g-1。结论:该方法准确,分析效率高,重复性好,适用于炎可宁片的质量控制。Objective:To establish an UPLC quantitative analysis method for the simultaneous determination of 13 components in Yankening tablets,including phellodendrine,coptisine,baicalin,palmatine,berberine,wogonoside,baicalein,aloe-emodin,rhein,wogonin,emodin,chrysophanol,physcion.Methods:Agilent Eclipse Plus C_(18)(100 mm×2.1 mm,1.8μm)column was used with acetonitrile-0.1%phosphoric acid as mobile phase,and gradient elution at a flow rate of 0.3 mL·min^(-1).The detection wavelengths were 210 nm and 254 nm.The column temperature was 40℃.Results:Phellodendrine hydrochloride,coptisine hydrochloride,palmatine hydrochloride,berberine hydrochloride,baicalin,wogonoside,baicalein,aloe-emodin,rhein,wogonin,emodin,chrysophanol,physcion showed good linear relationships within their concentration range of 0.97-48.63μg·mL-1,0.95-47.52μg·mL-1,0.86-43.15μg·mL-1,0.86-43.19μg·mL-1,0.89-44.37μg·mL-1,1.00-49.84μg·mL-1,1.02-51.01μg·mL-1,0.97-48.31μg·mL-1,0.99-49.50μg·mL-1,1.04-51.80μg·mL-1,1.00-50.04μg·mL-1,1.00-49.80μg·mL-1,1.01-50.64μg·mL-1.The average recoveries(n=6)were 95.2%,96.7%,95.9%,98.3%,94.1%,97.6%,99.2%,96.6%,95.5%,97.2%,97.0%,97.8%,98.7%,RSD values were all less than 2.0%.The contents of the 13 chemical components in 3 batches of samples were 1.367-1.488 mg·g-1(calculated as phellodendrine hydrochloride),0.378-0.412 mg·g-1(calculated as coptisine hydrochloride),4.611-5.505 mg·g-1,0.324-0.407 mg·g-1(calculated as palmatine hydrochloride),3.665-3.878 mg·g-1(calculated as berberine hydrochloride),1.107-1.682 mg·g-1,0.392-0.941 mg·g-1,0.076-0.105 mg·g-1,0.097-0.116 mg·g-1,1.059-1.213 mg·g-1,0.149-0.167 mg·g-1,0.213-0.239 mg·g-1,0.047-0.059 mg·g-1.Conclusion:The method is accurate,high analysis efficiency,good repeatability,it can be used to control the quality of Yankening tablets.

关 键 词：炎可宁片 超高效液相色谱法 消炎止痢 黄柏碱 黄连碱 黄芩苷 巴马汀 小檗碱 汉黄芩苷 黄芩素 芦荟大黄素 大黄酸 汉黄芩素 大黄素 大黄酚 大黄素甲醚 含量测定 质量控制 

分 类 号：R917[医药卫生—药物分析学]