基于非基因工程技术的细胞表面修饰策略研究进展  

Research Progress on Cell Surface Modification Strategies Based on Nongenetic Engineering Technologies

作　　者：毕瑞[1] 武江波 马春靖 BI Rui;WU Jiangbo;MA Chunjing(Department of Pharmacy,Tangshan People's Hospital,Hebei Tangshan 063000,China;Adverse Drug Reaction Monitoring Center,Market Supervision Administration of Tangshan,Hebei Tangshan 063000,China)

机构地区：[1]唐山市人民医院药学部,河北唐山063000 [2]河北省唐山市市场监督管理局,药品不良反应监测中心,河北唐山063000

出　　处：《生物技术进展》2024年第4期555-565,共11页Current Biotechnology

基　　金：河北省唐山市科技计划项目(14130256a);河北省药学会临床药学专项科研项目(YX201506)。

摘　　要：细胞表面成分与结构在维持细胞内代谢环境稳定、控制细胞内外物质交换和促进细胞间通讯方面发挥着至关重要的作用,因此,调节或改变细胞表面成分对研究细胞命运具有重要意义。基因工程是目前调节细胞表面成分最常用的技术,但是由于这种方法具有转染效率低、存在突变风险等问题,其应用范围仍然有限。相比之下,近些年发展起来的多种非基因工程细胞表面修饰技术,具有简便、易操作、适用范围广等优势,为调控细胞生命活动、赋予细胞新的性质和功能提供了更多新的选择,因而其在基础生物学研究和新药研发中具有广泛应用前景。对目前非基因工程技术的原理、特点以及在细胞治疗领域的应用等进行了综述和讨论,期望有助于深入了解这一新技术及其在生物医学领域中的应用。The cell surface plays a crucial role in maintaining a stable intracellular metabolic environment,controlling the exchange of substances inside and outside the cell,and facilitating intercellular communication.Therefore,the regulation or alteration of cell surface components is of significant importance in studying cellular fate.While genetic engineering remains the most commonly used technique for modulating cell surface components,its application is limited due to challenges such as low transfection efficiency and the risk of mutations.In contrast,various non-genetic engineering strategies developed in recent years offer new alternatives for regulating cellular activities,imparting cell with novel properties,and enhancing functionalities without the complexities associated with genetic manipulations.These non-genetic engineering approaches are characterized by their simplicity,ease of operation,and broad applicability,making them promising in fundamental biological research and pharmaceutical development.This paper provided a comprehensive review and discussion of the principles and characteristics of current non-genetic engineering strategies for cell surface modification,along with their applications in the field of cell therapy,aiming to offer insights into these strategies,develop new methodologies,and expand their biomedical applications.

关 键 词：非基因工程 细胞表面修饰技术 细胞治疗 新药研发 

分 类 号：Q953[生物学—动物学]