具有骨样微环境的压电催化可控矿化支架实现内源性骨再生  

Piezocatalytically-induced controllable mineralization scaffold with bone-like microenvironment to achieve endogenous bone regeneration

作　　者：崔希 胥玲玲 单义珠 李佳宣 吉健颖 汪恩贵 张葆堃 文晓舟 白源 罗聃 陈春英 李舟 Xi Cui;Lingling Xu;Yizhu Shan;Jiaxuan Li;Jianying Ji;Engui Wang;Baokun Zhang;Xiaozhou Wen;Yuan Bai;Dan Luo;Chunying Chen;Zhou Li(Beijing Key Laboratory of Micro-nano Energy and Sensor,Beijing Institute of Nanoenergy and Nanosystems,Chinese Academy of Sciences,Beijing 101400,China;School of Nanoscience and Engineering,University of Chinese Academy of Sciences,Beijing 100049,China;New Cornerstone Science Laboratory,CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience,National Center for Nanoscience and Technology,Beijing 100190,China)

机构地区：[1]Beijing Key Laboratory of Micro-nano Energy and Sensor,Beijing Institute of Nanoenergy and Nanosystems,Chinese Academy of Sciences,Beijing 101400,China [2]School of Nanoscience and Engineering,University of Chinese Academy of Sciences,Beijing 100049,China [3]New Cornerstone Science Laboratory,CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience,National Center for Nanoscience and Technology,Beijing 100190,China

出　　处：《Science Bulletin》2024年第12期1895-1908,共14页科学通报（英文版）

基　　金：supported by Beijing Natural Science Foundation(L212010);the National Natural Science Foundation of China(T2125003,52372174);the New Cornerstone Science Foundation,Major Instrument Project of the National Natural Science Foundation of China(22027810);the National Key Research and Development Program of China(2022YFB3804703,2022YFE0111700,2021YFA1200900,2021YFB3201204,2022YFB3205602)。

摘　　要：Orderly hierarchical structure with balanced mechanical,chemical,and electrical properties is the basis of the natural bone microenvironment.Inspired by nature,we developed a piezocatalytically-induced controlled mineralization strategy using piezoelectric polymer poly-L-lactic acid(PLLA)fibers with ordered micro-nano structures to prepare biomimetic tissue engineering scaffolds with a bone-like microenvironment(pcm-PLLA),in which PLLA-mediated piezoelectric catalysis promoted the in-situ polymerization of dopamine and subsequently regulated the controllable growth of hydroxyapatite crystals on the fiber surface.PLLA fibers,as analogs of mineralized collagen fibers,were arranged in an oriented manner,and ultimately formed a bone-like interconnected pore structure;in addition,they also provided bone-like piezoelectric properties.The uniformly sized HA nanocrystals formed by controlled mineralization provided a bone-like mechanical strength and chemical environment.The pcm-PLLA scaffold could rapidly recruit endogenous stem cells,and promote their osteogenic differentiation by activating cell membrane calcium channels and PI3K signaling pathways through ultrasound-responsive piezoelectric signals.In addition,the scaffold also provided a suitable microenvironment to promote macrophage M2 polarization and angiogenesis,thereby enhancing bone regeneration in skull defects of rats.The proposed piezocatalytically-induced controllable mineralization strategy provides a new idea for the development of tissue engineering scaffolds that can be implemented for multimodal physical stimulation therapy.

关 键 词：Piezoelectric catalysis Controllable mineralization BIOMIMETIC Bone repair BIODEGRADABLE 

分 类 号：R318[医药卫生—生物医学工程] O643.36[医药卫生—基础医学] TQ342[理学—物理化学]