基于转录组测序分析茯苓多糖对糖尿病大鼠创面愈合的影响  

The effect of Poria cocos polysaccharide on wound healing in diabetic rats analyzed based on RNA-sequencing

作　　者：彭珍雪 周芝森 刘璨宇 张选奋[2] PENG Zhenxue;ZHOU Zhisen;LIU Canyu;ZHANG Xuanfen(The Second Clinical Medical School of Lanzhou Universitu,Lanzhou 730030,China)

机构地区：[1]兰州大学第二临床医学院,甘肃兰州730030 [2]兰州大学第二医院整形外科,甘肃兰州730030

出　　处：《中国美容整形外科杂志》2024年第7期425-430,共6页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的 基于转录组测序分析茯苓多糖(poria cocos polysaccharide,PCP)对糖尿病大鼠创面愈合的影响。方法 自2023年9—10月,兰州大学第二医院整形外科将24只SPF级SD雄性大鼠,随机分为糖尿病(diabetic control, DC)组、正常对照(normal control, NC)组及PCP组,8只/组。前期预实验表明,1%PCP水溶液可促进糖尿病大鼠创面愈合。建立创面模型后,PCP组创面予1%PCP水溶液湿敷,NC组及DC组予生理盐水湿敷,1次/d。于术后第7天每组各随机抽取5只大鼠,计算创面愈合率,切取创面组织,行HE及Masson染色,观察各组大鼠创面组织病理学变化,行转录组测序(RNA-sequencing,RNA-seq),筛选出NC、DC组及DC、PCP组显著差异基因,进行京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes, KEGG)富集分析、基因本体(gene ontology, GO)富集分析及基因集富集分析(gene set enrichment analysis, GSEA)。每组余下各3只大鼠于术后第14天拍照记录、计算创面愈合率、行HE及Masson染色。结果 术后第7、14天,各组大鼠创面逐渐缩小,NC组创面愈合情况最佳,DC组最差,PCP组介于DC、NC组之间。术后第7、14天,HE染色显示,同一时间点,DC组有大量炎症细胞浸润,NC组有少量炎症细胞浸润,PCP组介于NC、DC组之间,术后第14天各组炎症细胞较术后第7天减少。KEGG、GO富集分析显示,DC组与NC组差异基因主要富集在c AMP、cGMP-PKG等信号通路,参与角化、上皮发育、炎症反应等生物学过程。PCP组与DC组差异基因主要富集在PI3K-Akt、Calcium等信号通路,参与新生血管的调控、上皮细胞发育、内皮细胞增殖的调节等生物学过程。GSEA分析显示,DC、NC组微小差异基因富集在白介素-17(interleukin-17, IL-17)、核因子-κB(nuclear factor kappa-B,NF-κB)、钙(Calcium)、肿瘤坏死因子(tumor necrosis facto,TNF)等信号通路,DC、PCP组富集在IL-17、cGMP-PKG等信号通路。结论 高糖可能通过c AMP、cGMP-PKG等信号�Objective To analyze the effect of poria cocos polysaccharide(PCP)on wound healing in diabetic rats based on RNA-seq.Methods From September to October 2023,24 SPF SD male rats were randomly divided into diabetic control(DC)group,normal control(NC)group and PCP group(8 rats in each group)in Department of Plastic Surgery,The Second Hospital of Lanzhou Univer-sitv.Previous experiments showed that 1%PCP aqueous solution could promote wound healing in diabetic rats.Wounds in PCP group were treated with 1%PCP aqueous solution on wet compress after operation,while wounds in normal control group and diabetic group were treated with normal saline wet compress.On the 7th day after operation,5 rats were randomly selected from each group to calculate the wound healing rate,and the wound tissue was cut and stained with HE to observe the pathological changes of the wound tissue in each group.RNA-seq was performed to screen out the significant differential genes in NC,DC group,and DC,PCP group,kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis,gene ontology(Ontology)analysis were performed(GO)enrichment analysis and Gene set enrichment analysis(GSEA)were performed.The remaining 3 rats in each group were photographed and recorded on the 14th day after op-eration,the wound healing rate was calculated,HE and Masson staining was performed.Results On the 7th and 14th day after injury,the wound of rats in each group was gradually reduced.The wound healing of NC group was the best,DC group was the worst,and PCP group was between DC group and NC group.HE staining showed that there were a large number of inflammatory cells infiltration in DC group and a small number of inflammatory cells infiltration in NC group at the same time point.The inflammatory cells in PCP group were between NC group and DC group.KEGG and GO enrichment analysis showed that the significantly different genes between DC group and NC group were mainly enriched in cAMP,cGMP-PKG and other signaling pathways.It is involved in biological processes such as

关 键 词：茯苓多糖 创面愈合 转录组测序 糖尿病 

分 类 号：R285.5[医药卫生—中药学]