人参UDP-阿拉伯糖变位酶基因电子克隆及分析  

In Silico Cloning and Analysis of UDP-arabinopyranose Mutases Gene in Panax Ginseng

作　　者：田鹏宇 宋敏丽[1] 张义茹 TIAN Pengyu;SONG Minli;ZHANG Yiru(College of Biological Sciences and Technology,Taiyuan Normal University,Jinzhong Shanxi 030619,China)

机构地区：[1]太原师范学院生物科学与技术学院,山西晋中030619

出　　处：《太原师范学院学报（自然科学版）》2024年第2期64-71,共8页Journal of Taiyuan Normal University:Natural Science Edition

摘　　要：UDP-阿拉伯糖变位酶是阿拉伯糖合成过程的关键酶,在植物形态建成和逆境胁迫响应过程中起着重要的作用.UDP-阿拉伯糖变位酶可使UDP-Araf和UDP-Arap相互转换,参与包括阿拉伯糖在内的多糖合成.以玉米UDP-阿拉伯糖变位酶氨基酸序列作为探针,通过电子克隆的方式,对人参EST数据库进行检索,获得具有高度同源性的cDNA序列,采用DNAMAN软件对其进行拼接和组装,最终获得一条长为1445 bp的cDNA序列.利用相关软件对人参UDP-阿拉伯糖变位酶基因进行生物信息学分析.结果表明:人参UDP-阿拉伯糖变位酶蛋白为稳定的亲水性蛋白,该蛋白上的磷酸化修饰有33处,无跨膜螺旋区,不存在信号肽,为非分泌蛋白.该蛋白含有无规则卷曲、α螺旋、延伸链、β折叠二级结构,其中无规则卷曲结构最多.人参UDP-阿拉伯糖变位酶的氨基酸序列与凤仙花同源性较高,与石榴花同源性较低.分析结果能为人参后续相关研究提供一定的参考依据.UDP-arabinopyranose mutase is a key enzyme in the process of arabinose synthe-sis,which plays an important role in plant morphogenesis and response to adversity stress.UDP-arabinopyranose mutase can interconvert UDP-Araf and UDP-Arap and participate in the biosynthesis of polysaccharides including arabinose.Using the amino acid sequence of the UDP-arabinopyranose mutase of Maize as a probe,the EST database of Panar ginseng was searched by silicon cloning to obtain a cDNA sequence with a high degree of homology,which was spliced and assembled using the DNAMAN software,and a cDNA sequence with a length of 1445 bp was finally obtained.Bioinfor-matics analysis of the UDP-arabinopyranose mutase gene of Panac ginseng was carried out using the relevant softwares.The results showed that the UDP-arabinopyranose mutase protein of Panac gin-seng is a stable hydrophilic protein,there are 33 phosphorylation modifications on the protein,no trans-membrane helical region,no signal peptide,and it is a non-secretory protein.The protein contains ir-regularly coiled,α-helix,extended chain,andβ-folded secondary structures,of which the irregularly coiled structure is the most abundant.The amino acid sequence of the UDP-arabinopyranose mutase of Panar ginseng has a higher homology with Fenugreek and lower with Pomegranate.The analyzed re-sults can provide certain reference basis for the subsequent related studies on Panax ginseng.

关 键 词：人参 UDP-阿拉伯糖变位酶 电子克隆 生物信息学分析 

分 类 号：Q943.2[生物学—植物学] S567.51[农业科学—中草药栽培]