Microplastic exposure disturbs sleep structure,reduces lifespan,and decreases ovary size in Drosophila melanogaster  

作　　者：Wei Yan Zi-Jie Li Zi-Yi Lin Shu-Qin Ji William Ka Fai Tse Zhi-Qiang Meng Chang Liu Lei Li 

机构地区：[1]Shenzhen-Hong Kong Institute of Brain Science,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong 518000,China [2]CAS Key Laboratory of Brain Connectome and Manipulation,the Brain Cognition and Brain Disease Institute,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong 518000,China [3]Laboratory of Developmental Disorders and Toxicology,Center for Promotion of International Education and Research,Faculty of Agriculture,Kyushu University,Fukuoka,Fukuoka 819-0395,Japan [4]Shenzhen Key Laboratory of Drug Addiction,the Brain Cognition and Brain Disease Institute,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong 518000,China [5]Guangdong Provincial Key Laboratory of Brain Connectome and Behavior,Brain Cognition and Brain Disease Institute,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen,Guangdong 518000,China

出　　处：《Zoological Research》2024年第4期805-820,共16页动物学研究（英文）

基　　金：Key Collaborative Research Program of the Alliance of International Science Organizations(ANSO-CR-KP-2021-12 to L.L.);National Natural Science Foundation of China(32071009,32371063,82341248 to C.L.,31971072 and 32171154 to L.L.);Guangdong Basic and Applied Basic Research Foundation(2024A1515011500 to C.L.);Shenzhen Science Technology and Innovative Commission(SZSTI JCYJ20180508152336419 to L.L.and GJHZ20200731095406021 to S.J.)。

摘　　要：The organ-specific toxicity resulting from microplastic(MP)exposure has been extensively explored,particularly concerning the gut,liver,testis,and lung.However,under natural conditions,these effects are not restricted to specific organs or tissues.Investigating whether MP exposure presents a systemic threat to an entire organism,impacting factors such as lifespan,sleep,and fecundity,is essential.In this study,we investigated the effects of dietary exposure to two different doses of MPs(1–5μm)using the terrestrial model organism Drosophila melanogaster.Results indicated that the particles caused gut damage and remained within the digestive system.Continuous MP exposure significantly shortened the lifespan of adult flies.Even short-term exposure disrupted sleep patterns,increasing the length of daytime sleep episodes.Additionally,one week of MP exposure reduced ovary size,with a trend towards decreased egg-laying in mated females.Although MPs did not penetrate the brain or ovaries,transcriptome analysis revealed altered gene expression in these tissues.In the ovary,Gene Ontology(GO)analysis indicated genotoxic effects impacting inflammation,circadian regulation,and metabolic processes,with significant impacts on extracellular structure-related pathways.In the brain,GO analysis identified changes in pathways associated with proteolysis and carbohydrate metabolism.Overall,this study provides compelling evidence of the systemic negative effects of MP exposure,highlighting the urgent need to address and mitigate environmental MP pollution.

关 键 词：Microplastics SLEEP LIFESPAN REPRODUCTION Risk assessment Drosophila melanogaster 

分 类 号：X505[环境科学与工程—环境工程] X503