寄主转换对南亚果实蝇不同虫态主要酶活性的影响  

Effects of host transfer on main enzymes activities of Bactrocera tau((Walker))at different developmental stages

作　　者：翟雨桐 张彬 于永浩[3] 曾宪儒[3] 龙秀珍[3] 韦德卫[3] 何瞻 高旭渊[3] 周忠实 ZHAI Yu-tong;ZHANG Bin;YU Yong-hao;ZENG Xian-ru;LONG Xiu-zhen;WEI De-wei;HE Zhan;GAO Xu-yuan;ZHOU Zhong-shi(College of Plant Health&Medicine,Qingdao Agricultural University,Qingdao,Shandong 266109,China;Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Plant Protection Research Institute,Guangxi Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control on Fruits and Vege-tables in South China,Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests,Nanning,Guangxi 530007,China)

机构地区：[1]青岛农业大学植物医学学院,山东青岛266109 [2]中国农业科学院植物保护研究所,北京100081 [3]广西农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广西作物病虫害生物学重点实验室,广西南宁530007

出　　处：《南方农业学报》2024年第5期1375-1383,共9页Journal of Southern Agriculture

基　　金：国家自然科学基金青年科学基金项目(32102205);广西农业科学院科技发展基金项目(桂农科2021JM71)。

摘　　要：【目的】探索取食罗汉果[Siraitia grosvenorii(Swingle)C.Jeffery]的南亚果实蝇[Bactrocera tau(Walker)]在寄主转换后不同虫态体内主要酶活性的变化,以期从生理生化角度解析南亚果实蝇的寄主适应机制,为南亚果实蝇的有效防控提供理论依据。【方法】以采集自罗汉果果园并在室内连续用罗汉果饲养2年的南亚果实蝇种群为试验材料,分别用南瓜、丝瓜、黄瓜和罗汉果4种寄主转接室内饲养的南亚果实蝇,对取食不同寄主后的南亚果实蝇5日龄幼虫、2日龄蛹和7日龄成虫体内抗氧化酶[超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)]、解毒酶[谷胱甘肽转移酶(GST)和羧酸酯酶(CarE)]、消化酶[胃蛋白酶、α-淀粉酶和脂肪酶(LPS)]和水解酶[海藻糖酶(THL)]活性进行测定。【结果】取食不同寄主的南亚果实蝇体内SOD活性均在成虫期显著高于其他虫态(P<0.05,下同);取食罗汉果的幼虫期和成虫期南亚果实蝇CAT活性显著高于取食其他寄主处理,且取食不同寄主的南亚果实蝇CAT活性在成虫期低于(除取食南瓜的成虫期南亚果实蝇CAT活性稍高于蛹期外)或显著低于其他虫态。各虫态取食罗汉果的南亚果实蝇GST活性均高于或显著高于同龄期取食其他寄主处理,取食不同寄主的南亚果实蝇GST活性均在成虫期显著低于其他虫态;取食罗汉果的幼虫期和蛹期南亚果实蝇CarE活性高于或显著高于取食其他寄主处理。同一虫态,取食罗汉果的南亚果实蝇α-淀粉酶活性均高于或显著高于取食其他寄主处理;取食南瓜的幼虫期南亚果实蝇LPS活性高于其他寄主和虫态;成虫期取食不同寄主的南亚果实蝇THL活性差异显著,其中取食南瓜的南亚果实蝇THL活性显著高于取食其他寄主处理,取食罗汉果的南亚果实蝇THL活性则显著低于取食其他寄主处理。【结论】南亚果实蝇对罗汉果的适应性更高,与南瓜、丝瓜和�【Objective】The study investigated the changes in major enzyme activities in different developmental stages of Bactrocera tau(Walker)fed on Siraitia grosvenorii(Swingle)C.Jeffery after host transfer,aiming to elucidate the host adaptation of B.tau from physiological and biochemical mechanism,and therefore provide a theoretical basis for its effective control.【Method】The B.tau population collected from a S.grosvenorii orchard and fed with S.grosvenorii indoors for 2 years was transferred to four different host plants:pumpkin,loofah,cucumber,and S.grosvenorii.Then measured the activities of antioxidant enzymes[superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)],detoxifying enzymes[glutathione S-transferase(GST),carboxylesterase(CarE)],digestive enzymes[pepsin,α-amylase,lipase(LPS)],and hydrolytic enzyme trehalase(THL)in 5-day-old larvae,2-day-old pupae and 7-day-old adults.【Result】The SOD activity of B.tau feeding on different hosts was significantly higher in the adult stage than in other developmental stages across all host plants(P<0.05,the same below).The CAT activity was significantly higher in the larval and adult stage feeding on S.grosvenorii than those feeding on other hosts.However,the CAT activity in the adult stage of B.tau feeding on different hosts was lower(except that the CAT activity in adults feeding on pumpkin was slightly higher than that in pupae)or significantly lower than those in other developmental stages.Similarly,B.tau feeding on S.grosvenorii showed higher or significantly higher GST activity at the same stage compared to those feeding on other hosts,while B.tau adults feeding on all host plants exhibi-ted significantly lower GST activity compared to other stages.The CarE activity in larval and pupal stages feeding on S.grosvenorii was higher or significantly higher than those on other hosts.Theα-amylase activity of B.tau feeding on S.grosvenorii was higher or significantly higher than those on other hosts at the same stage.The LPS activity in larvae of B.tau feeding on

关 键 词：南亚果实蝇 罗汉果 寄主转换 优势寄主 酶活性 

分 类 号：S433[农业科学—农业昆虫与害虫防治]