不同激活剂对富血小板血浆生长因子的影响  

Effects of different activators on platelet-rich plasma growth factors

作　　者：刘建香[1] 冯星星[2] 王淑霞[1] 周蓉[1] 吕孟兴[1] 屈柯暄 Liu Jianxiang;Feng Xingxing;Wang Shuxia;Zhou Rong;Lyu Mengxing;Qu Kexuan(Department of Blood Transfusion,Kunming Children’s Hospital,Kunming 650028,Yunnan Province,China;Department of Clinical Laboratory,Kunming Children’s Hospital,Kunming 650028,Yunnan Province,China;Kunming Medical Technology Center for Immunology and Molecular Diagnosis of Children’s Blood Diseases,Kunming 650028,Yunnan Province,China)

机构地区：[1]昆明市儿童医院输血科,云南省昆明市650028 [2]昆明市儿童医院检验科,云南省昆明市650028 [3]昆明市儿童血液疾病免疫与分子诊断医学技术中心,云南省昆明市650028

出　　处：《中国组织工程研究》2025年第10期2067-2073,共7页Chinese Journal of Tissue Engineering Research

基　　金：昆明市卫生科技人才培养项目[2021-SW(省)-024],项目负责人:屈柯暄;昆明市卫生科技人才培养项目[2022-SW(技术)-007],项目参与人:冯星星、屈柯暄;昆明市卫生健康委员会卫生科研科课题项目(2022-06-04-003),项目参与人:屈柯暄、吕孟兴、刘建香;昆明市卫生健康委员会卫生科研科课题项目(2023-11-01-022),项目负责人:刘建香。

摘　　要：背景:生长因子是富血小板血浆在临床治疗中发挥作用的关键效应分子,不同激活剂激活富血小板血浆后生长因子浓度存在差异,是影响临床疗效的重要因素。目的:分析不同激活剂对富血小板血浆中生长因子质量浓度的影响。方法:招募12名健康志愿者,采集EDTA-K2抗凝静脉血,应用二次离心法制备富血小板血浆。比较静脉血与富血小板血浆中生长因子的质量浓度差异。将富血小板血浆分别与4种激活剂(生理盐水、凝血酶、葡萄糖酸钙、葡萄糖酸钙+凝血酶)按照体积比10∶1混匀,置于37℃恒温水浴箱孵育30 min,离心后提取上清液,检测生长因子质量浓度;利用血琼脂平板检测上清液中的细菌生长情况;采用Pearson相关分析不同激活剂与富血小板血浆中生长因子质量浓度的相关性,血小板计数值与富血小板血浆中生长因子质量浓度的相关性。结果与结论:(1)富血小板血浆中血小板衍生生长因子BB、血小板衍生生长因子AB、血管内皮生长因子、表皮生长因子的质量浓度分别是静脉血中对应生长因子质量浓度的8.7,22.2,2.3,2.8倍(P <0.05);(2)与生理盐水组比较,凝血酶组、葡萄糖酸钙组、葡萄糖酸钙+凝血酶组中血小板衍生生长因子BB、血小板衍生生长因子AB、血管内皮生长因子、表皮生长因子质量浓度升高(P <0.05);凝血酶组、葡萄糖酸钙组血小板衍生生长因子BB质量浓度高于葡萄糖酸钙+凝血酶组(P <0.05),凝血酶组血小板衍生生长因子AB质量浓度高于葡萄糖酸钙组、葡萄糖酸钙+凝血酶组(P <0.05),凝血酶组表皮生长因子质量浓度低于葡萄糖酸钙组、葡萄糖酸钙+凝血酶组(P <0.05);(3)血琼脂平板实验结果显示4组上清液中均无细菌生长;(4)Pearson相关分析显示,富血小板血浆中血小板衍生生长因子BB质量浓度与凝血酶存在正向强相关性(r=0.683,P <0.05),血管内皮生长因子质量浓度与凝血酶、BACKGROUND:Growth factor is the key effect molecule that plays a role in platelet-rich plasma in clinical treatment.There are differences in the concentration of growth factor after different activators activate platelet-rich plasma,which is an important factor affecting clinical efficacy.OBJECTIVE:To analyze the influence of different activators on the mass concentration of growth factors in platelet-rich plasma.METHODS:Totally 12 healthy volunteers were recruited to collect EDTA-K2 anticoagulant venous blood.Secondary centrifugation was used to prepare platelet-rich plasma.The difference in mass concentrations of growth factors was compared between venous blood and platelet-rich plasma.The platelet-rich plasma was mixed with four activators(normal saline,thrombin,calcium gluconate,calcium gluconate+thrombin)according to the volume ratio of 10:1,and incubated in a constant temperature water bath at 37°C for 30 minutes.After centrifugation,the supernatant was extracted and the mass concentration of growth factor was detected.The bacterial growth in supernatant was measured by blood agar plate.Pearson correlation was used to analyze the correlation between different activators and the mass concentration of growth factor in platelet-rich plasma,and the correlation between the value of thrombocytometer and the mass concentration of growth factors in platelet-rich plasma.RESULTS AND CONCLUSION:(1)The mass concentrations of platelet-derived growth factor-BB,platelet-derived growth factor-AB,vascular endothelial growth factor,and epidermal growth factor in platelet-rich plasma were 8.7,22.2,2.3,and 2.8 times of those in venous blood,respectively(P<0.05).(2)Compared with normal saline group,the mass concentrations of platelet-derived growth factor BB,platelet-derived growth factor AB,vascular endothelial growth factor,and epidermal growth factor were increased in the thrombin group,calcium gluconate group,and calcium gluconate+thrombin group(P<0.05).The mass concentration of platelet-derived growth factor BB in the thr

关 键 词：富血小板血浆 凝血酶 葡萄糖酸钙 血小板衍生生长因子BB 血小板衍生生长因子AB 血管内皮生长因子 表皮生长因子 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R496