两样本孟德尔随机化分析肠道菌群相对丰度与畸形精子症的因果关系  

Causal relationship between relative abundance of gut microbiota and teratozoospermia:A two-sample Mendelian randomization analysis

作　　者：郝晓慧 马瑞敏 马思成 刘稳榜 张宸铭[1] 于雯霖 胡静[1] 王祖龙[2] HAO Xiao-hui;MA Rui-min;MA Si-cheng;LIU Wen-bang;ZHANG Chen-ming;YU Wen-lin;HU Jing;WANG Zu-long(Henan University of Chinese Medicine,Zhengzhou,Henan 450046,China;Department of Andrology,The First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou,Henan 450003,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南中医药大学第一附属医院男科,河南郑州450003

出　　处：《中华男科学杂志》2024年第5期387-396,共10页National Journal of Andrology

基　　金：中国博士后科研基金第16批特别资助(2023T160201);中国博士后基金面上项目(2022M721068);国家级大学生创新创业训练计划(202310471011)。

摘　　要：目的:探究肠道菌群与畸形精子症的潜在因果关系。方法:从全基因组关联研究(GWAS)数据库中选择肠道菌群与畸形精子症的GWAS数据。肠道菌群作为暴露因素,工具变量(IVs)从MiBioGen联盟发布的18340例参与者的GWAS中确定。结局变量来自欧洲的畸形精子症GWAS数据,总样本量85716例,其中915例患者,209006例对照。采用逆方差加权法(IVW)、MR-Egger回归和加权中位数法(WME)进行两样本孟德尔随机化分析,以β值来评价肠道菌群与畸形精子症之间的因果关系。结果:丹毒丝菌纲、丹毒丝菌科、链球菌科、粪球菌属Coprococcus1、瘤胃菌外UCG009属、链球菌属、梭状芽胞杆菌目、丹毒丝菌目与畸形精子症的风险增高存在因果关系,紫单胞菌科与畸形精子症的风险降低存在因果关系。结论:丹毒丝菌纲、丹毒丝菌科、链球菌科、粪球菌属Coprococcus1、瘤胃菌外UCG009属、链球菌属、梭状芽胞杆菌目、丹毒丝菌目可能是引起畸形精子症的原因之一,紫单胞菌科可能对精子的形态具有保护作用,能够减少畸形精子症的发生风险。Objective:To explore the potential causal relationship between gut microbiota and teratozoospermia.Methods:We searched the database of Genome-Wide Association Study(GWAS)for gut microbiota-and teratozoospermia-related data.We used gut microbiota as an exposure factor,determined the instrumental variables according to the GWAS data on 18340 participants released by the MiBioGen Alliance,and derived the outcome variables from the European data on teratozoospermia,with a sample size of 85716,including 915 cases and 209006 controls.Using inverse-variance weighting(IVW),MR-Egger regression and the weighted median estimator(WME),we performed two-sample Mendelian randomization(MR)analysis on the retrieved data,and estimated the causal relationship between gut microbiota and teratozoospermia based on theβvalue.Results:Two-sample MR analysis indicated that the class Erysipelotrichia,family Erysipelotrichaceae,family Streptococcaceae,genus Coprococcusl,genus Ruminococcaceae UCG009,genus Streptococcus,order Clostridialesm and order Erysipelotrichales were causally related with the increased risk,while the family Porphyromonadaceae with the decreased risk of teratozoospermia.Conclusion:The class Erysipelotrichia,family Erysipelotrichaceae,family Streptococcaceae,genus Coprococcusl,genus Ruminococcaceae UCG009,genus Streptococcus,order Clostridialesm and order Erysipelotrichales are one of the causes of teratozoospermia,related to the increased risk of the condition,while the family Porphyromonadaceae has a protective effect on sperm morphology,reducing the risk of teratozoospermia.

关 键 词：畸形精子症 肠道菌群 孟德尔随机化研究 

分 类 号：R698.2[医药卫生—泌尿科学]