推拿“委中”穴对坐骨神经慢性压迫性损伤模型大鼠海马CA3区突触可塑性的影响  

Effects of Tuina at "Weizhong" (BL 40) on Synaptic Plasticity in the Hippocampal CA3 Region in Chronic Sciatic Nerve Compression Injury Model Rats

作　　者：黄红叶 陈水金 陈乐春 张幻真 蒋晶晶 江煜 陈进城 林志刚 HUANG Hongye;CHEN Shuijin;CHEN Lechun;ZHANG Huanzhen;JIANG Jingjing;JIANG Yu;CHEN Jincheng;LIN Zhigang(Rehabilitation Hospital affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou,350003)

机构地区：[1]福建中医药大学附属康复医院,福建省福州市350003

出　　处：《中医杂志》2024年第15期1602-1610,共9页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(82174523,82105039,82205303);福建省杰出青年基金(2023J06037);福建省自然科学基金(2020J01760);福建省卫生健康中青年领军人才研修培养项目。

摘　　要：目的 从海马突触可塑性角度探讨推拿委中穴缓解坐骨神经痛的可能作用机制。方法 60只SD大鼠随机分为假手术组、模型组、推拿组、MK-801组、MK-801+推拿组,每组12只。所有大鼠侧脑室置管术后,除假手术组外其余各组大鼠制备右侧坐骨神经慢性压迫性损伤模型。造模后第4天,推拿组大鼠开始推拿“委中”穴干预,每日1次持续10 min,共干预14天;MK-801组大鼠开始侧脑室注射0.25μg/μl的N-甲基-D天冬氨酸受体2B (NR2B)阻断剂地佐环平(MK-801) 0.5μl,每日1次,共14天。MK-801+推拿组大鼠在侧脑室注射MK-801 30 min后进行推拿干预,时间方法同上;模型组和假手术组大鼠不进行任何干预。大鼠造模前1天(基础值)及造模第4、10、14、18天进行自发痛行为评分和机械足反射阈值(PWTs)检测,第19天各组大鼠脑组织取材后,使用尼氏染色观察海马CA3区尼氏阳性细胞数量与形态,透射电镜观察海马CA3区突触数目、突触间隙、突触后致密物厚度、活性带长度与突触界面曲率,免疫荧光染色和免疫印迹法检测海马CA3区突触相关蛋白NR2B、突触后致密物95(PSD95)的表达。结果 与假手术组同时间比较,模型组造模后第4、10、14、18天时自发性疼痛评分显著增加,PWTs降低(P<0.05);与模型组比较,推拿组大鼠在造模第10、14、18天自发性疼痛评分显著下降,在造模第14、18天PWTs显著升高(P<0.05);与推拿组比较,MK-801+推拿组自发性疼痛评分在造模第10、14、18天升高,在造模第14、18天PWTs降低(P<0.05)。与假手术组比较,模型组大鼠海马CA3区神经元排列紊乱,尼氏阳性细胞数量、突触数目、突触后致密度厚度、活性带长度、突触界面曲率减小,突触间隙变宽,海马CA3区NR2B、PSD95免疫荧光阳性表达及免疫印迹蛋白表达减少(P<0.05)。与模型组比较,推拿组大鼠海马CA3区神经细胞排列密集,尼氏阳性细胞数量、突触数目、突触后致密�Objective To explore the possible mechanism of Tuina at Weizhong(BL 40)for relieving sciatica from the perspective of hippocampal synaptic plasticity.Methods Sixty SD rats were randomly divided into sham operation group,model group,Tuina group,MK-801 group,MK-801 plus Tuina group,12 rats in each group.After lateral ventricular cannulation,rats model with chronic compression injury of the right sciatic nerve were prepared in all groups except the sham operation group.On day 4 after modelling,rats in the Tuina group start Tuina at Weizhong(BL 40)for 10 mins once a day for a total of 14 days;rats in the MK-801 group started injecting with 0.25μg/μl of the N-methyl-D-aspartate receptor 2B(NR2B)blocker,dizocycline(MK-801),0.5μl of which was administered daily in the lateral ventricle for 14 days.Rats in the MK-801 plus Tuina group underwent Tuina after 30 mins when completing MK-801 injection in the lateral ventricle,in the same way as above;rats in the model group and the sham operation group did not undergo any intervention.Spontaneous pain behaviour scores and paw withdraw thresholds(PWTs)were examined on day 1(base value)before modelling and on day 4,10,14 and 18 after modelling;and on day 19,the brain tissues of the rats in each group were sampled and the number and morphology of the Nysted-positive cells in the hippocampal CA3 region were observed using Nysted staining;and the number of synapses,the thickness of postsynaptic dense material,the length of active band and the curvature of synaptic interface in hippocampal CA3 region were observed by transmission electron microscopy;and the expression of synapse-associated proteins NR2B and postsynaptic density protein-95(PSD95)in hippocampal CA3 was detected by immunofluorescence staining and immunoblotting.Results Compared with the same time in the sham operation group,spontaneous pain scores significantly increased and PWTs decreased on day 4,10,14,and 18 after modelling in the model group(P<0.05);compared with the model group,spontaneous pain scores in Tuina

关 键 词：坐骨神经痛 推拿 委中穴 海马 突触可塑性 N-甲基-D天冬氨酸受体2B 

分 类 号：R244.1[医药卫生—针灸推拿学]