2009-2024年山东省甲型H1N1流感病毒血凝素基因进化分析  

Molecular evolutionary of hemagglutinin gene of influenza A(H1N1)pdm09 virus in Shandong Province from 2009 to 2024

作　　者：赵志红 何玉洁 吴巨龙[2] 宋绍霞[2] 孙林[2] 李忠[2] 王显军[2] 寇增强[2] 温红玲 刘倜[2] Zhao Zhihong;He Yujie;Wu Julong;Song Shaoxia;Sun Lin;Li Zhong;Wang Xianjun;Kou Zengqiang;Wen Hongling;Liu Ti(Department of Microbiological Laboratory Technology,School of Public Health,Cheeloo College of Medicine,Key Laboratory of Emerging Infectious Diseases Control and Prevention and Biosafety in Universities of Shandong,Shandong University,Jinan 250012,China;Shandong Institute of Preventive Medicine,Shandong Center for Disease Control and Prevention,Jinan 250014,China)

机构地区：[1]山东大学齐鲁医学院公共卫生学院微生物检验学系,山东省"十四五"高等学校新发突发传染病防控与生物安全重点实验室,济南250012 [2]山东省疾病预防控制中心,山东省预防医学研究院,济南250014

出　　处：《中华微生物学和免疫学杂志》2024年第7期580-587,共8页Chinese Journal of Microbiology and Immunology

基　　金：山东省自然科学基金(ZR2021MH372);山东省公共卫生学会科研项目(SGWXH202301)。

摘　　要：目的描述并分析山东省A(H1N1)pdm09亚型流感病毒血凝素(hemagglutinin,HA)基因的多样性,探讨其遗传变异规律,为流感监测、疫情防控和疫苗株匹配度提供科学参考。方法从GISAID流感数据平台下载2009—2024年流感疫苗推荐株和各分支代表株的HA基因序列,与山东省分离的298株甲型H1N1流感病毒株HA基因序列进行系统发育分析和氨基酸位点变异分析。利用IQ-TREE在线工具的最大似然法(maximum likelihood,ML)构建系统进化树,利用MegAlign软件查看氨基酸位点变异,同时采用NetNGlyc 1.0在线软件预测HA基因潜在的糖基化位点。结果298株山东省甲型H1N1流感病毒的HA基因同源性为91.2%~100.0%,序列进化分支随时间推移亲缘关系渐远,但进化方向与其他省份大致相同。HA基因氨基酸变异在各年度均有发生,抗原决定簇是其主要变异区域。结论山东省2009—2024年流感病毒分离株的HA基因仍处于不断进化过程,持续监测流感病毒流行趋势和进化方向对预警流感病毒大流行至关重要。Objective To characterize and analyze the genetic variation of hemagglutinin(HA)of influenza A(H1N1)pdm09 subtype virus in Shandong Province,and explore the genetic variation patterns for providing reference for influenza monitoring,epidemic prevention and control,and vaccine strain selection.Methods HA gene sequences of the recommended strains of influenza vaccine from 2009 to 2024 and the representative strains of each branch were downloaded from the GISAID Influenza Data Platform,and were phylogenetically analyzed and characterized in terms of amino acid site variation with the HA gene sequences of 298 influenza A(H1N1)virus strains isolated from Shandong Province.A phylogenetic tree was constructed using the maximum likelihood(ML)method of the IQ-TREE online tool,and the amino acid site variants were viewed using MegAlign software.The potential glycosylation sites of the HA gene were predicted using the NetNGlyc 1.0 online software.Results The HA gene homology of the 298 influenza A(H1N1)viruses isolated in Shandong Province ranged from 91.2%to 100.0%.The evolutionary branches were gradually distantly related over time,but the direction of evolution was roughly the same as that in other provinces.Amino acid mutations in the HA occurred every year and most were found in the antigenic determinants.Conclusions The HA genes of influenza viruses isolated in Shandong Province from 2009 to 2024 are still in the process of continuous evolution,and continuous monitoring of the epidemiological trends and the evolutionary directions of influenza viruses is essential for early warning of influenza virus pandemics.

关 键 词：流感病毒A型 H1N1亚型 血凝素基因 遗传进化 氨基酸变异 

分 类 号：R511.7[医药卫生—内科学]