天津市儿童中人偏肺病毒的流行病学及分子特征分析  

Epidemiological and molecular characteristics of human metapneumovirus in children in Tianjin

作　　者：李蕊 郭丽茹[2] 孔梅[2] 邹明[2] 刘小畅 王涛 高梦雪 李晓燕[2] Li Rui;Guo Liru;Kong Mei;Zou Ming;Liu Xiaochang;Wang Tao;Gao Mengxue;Li Xiaoyan(School of Public Health,Tianjin Medical University,Tianjin 300070,China;Tianjin Key Laboratory of Pathogenic Microbiology of Infectious Disease,Institute of Microbiology,Tianjin Center for Disease Control and Prevention,Tianjin 300011,China;School of Life Sciences,Tianjin University,Tianjin 300072,China)

机构地区：[1]天津医科大学公共卫生学院,天津300070 [2]天津市疾病预防控制中心微生物检验检测所,天津市传染病病原微生物重点实验室,天津300011 [3]天津大学生命科学学院,天津300072

出　　处：《中华微生物学和免疫学杂志》2024年第7期588-595,共8页Chinese Journal of Microbiology and Immunology

基　　金：天津市卫生健康委重点项目(TJWJ2022ZD010);天津市科技局重点项目(20JCZDJC00130);天津市医学重点学科(专科)建设项目。

摘　　要：目的分析2020年10月—2021年3月以及2021年10月—2022年3月两个流感流行季天津地区人偏肺病毒(human metapneumovirus,hMPV)的流行病学特点及全基因组特征,丰富我国hMPV的全基因组数据库。方法收集流感样病例(influenza-like illness,ILI)患者的咽拭子样本共1040例,采用微流体芯片荧光定量PCR进行检测,对hMPV阳性样本采用RT-PCR扩增其全基因组,全基因组二代测序技术进行序列测定,使用CLC、DNAStar和MEGA等生物信息学软件进行序列组装,分析其核苷酸与氨基酸同源性并绘制系统发育树。结果1040例咽拭子样本中共检出25例hMPV阳性样本,检出率为2.40%,3~5岁年龄组的检出率最高,为3.71%(16/431)。在流感流行季中,hMPV流行高峰在12月份,其检出率为6.67%(12/180)。测序成功的12株序列中有7株为B2型、4株为A2b型、1株为B1型。其中G基因变异最大,有3株A2b型的G基因中存在111nt-dup的核苷酸重复。结论天津地区流感流行季中,hMPV的流行高峰为12月份,流行株以B2型为主,除G基因变异较大之外,其他基因较为稳定。Objective To investigate the epidemiological and genetic characteristics of human metapneumovirus(hMPV)in Tianjin during two influenza epidemic seasons from October 2020 to March 2021 and from October 2021 to March 2022,and enrich the whole genome database of hMPV in China.Methods A total of 1040 pharyngeal swab samples collected from patients with influenza-like illness(ILI)were analyzed using microfluidic chip fluorescence quantitative PCR.RT-PCR was used to amplify the whole genome in hMPV-positive samples,and the second-generation sequencing was performed for complete genome sequencing.Bioinformatics software including CLC,DNAStar,and MEGA was used for sequence assembly,nucleotide and amino acid homology analysis,and phylogenetic tree mapping.Results Among the 1040 samples,25 were positive for hMPV with a positive rate of 2.40%.The highest positive rate was observed in the age group of 3 to 5 years,reaching 3.71%(16/431).During the influenza epidemic seasons,the detection rate of hMPV peaked in December,reaching 6.67%(12/180).Twelve strains were successfully sequenced,and there were seven of type B2,four of type A2b,and one of type B1.More variations were detected in the G gene,with 111nt-dup sequence repeats observed in the G gene of three A2b strains.Conclusions The prevalence of hMPV peaks in December during the influenza epidemic seasons in Tianjin,with Type B2 being the predominant type.Except for the G gene with more mutations,other genes remain stable.

关 键 词：人偏肺病毒 流行特征 系统发育分析 呼吸道感染 

分 类 号：R725.1[医药卫生—儿科] R181.3[医药卫生—临床医学]