松杉灵芝发酵菌丝体中的三萜化合物  

Triterpenoids identified from the fermented mycelia of Ganoderma tsugae(Polyporales,Basidiomycota)

作　　者：郭晓宇 程池露 滕李铭 员瑗 张劲松[2] 潘新华 潘峰 戴玉成[3] 冯娜[2] GUO Xiaoyu;CHENG Chilu;TENG Liming;YUAN Yuan;ZHANG Jingsong;PAN Xinhua;PAN Feng;DAI Yucheng;FENG Na(College of Food Sciences&Technology,Shanghai Ocean University,Shanghai 201306,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,National Engineering Research Center of Edible Fungi,National R&D Center for Edible Fungal Processing,Key Laboratory of Edible Fungal Resources and Utilization(South),Ministry of Agriculture and Rural Affairs of the People’s Republic of China,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;State Key Laboratory of Efficient Production of Forest Resources,School of Ecology and Nature Conservation,Beijing Forestry University,Beijing 100083,China;Jiangxi Xiankelai Biotechnology Co.,Ltd.,Jiujiang 332000,Jiangxi,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海市农业科学院食用菌研究所、农业农村部南方食用菌资源利用重点实验室、国家食用菌工程技术研究中心、国家食用菌加工技术研发分中心、上海市农业遗传育种重点开放实验室,上海201403 [3]北京林业大学生态与自然保护学院林木资源高效生产全国重点实验室,北京100083 [4]江西仙客来生物科技有限公司,江西九江332000

出　　处：《菌物学报》2024年第7期124-134,共11页Mycosystema

基　　金：国家自然科学基金(U23A20142);上海市农业科学院卓越团队建设计划[沪农科卓(2022)003]。

摘　　要：通过中高压层析、薄层色谱层析、凝胶色谱层析等多种分离纯化技术,从松杉灵芝发酵菌丝体中制备得到15个羊毛甾烷型四环三萜化合物,通过核磁共振、质谱对化合物结构进行解析,分别鉴定为(22S,24E)-3-oxo-15α,22β-dihydroxylanosta-7,9(11),24-trien-26-oicacid(1)、(22S,24E)-3-oxo-15α-hydroxy-22β-acetoxylanosta-7,9(11),24-trien-26-oicacid(2)、(22S,24E)-3-oxo-15α,22β-diacetoxylanosta-7,9(11),24-trien-26-oicacid(3)、15-hydroxy-ganodericacidS(4)、ganodermicacidJb(5)、ganoderic acid P(6)、ganodermic acid Ja(7)、ganoderic acid X(8)、ganodermic acid TQ(9)、ganoderic acid Me(10)、ganodericacidMf(11)、ganodericacidY(12)、ganodericacidZ(13)、lanosta-7,9,(11),24-trien-3α-hydroxy-26-oic acid(14)和ganoderic acid S1(15)。以上15个化合物均为首次从松杉灵芝发酵菌丝体中分离获得。该结果丰富了灵芝属真菌发酵产物的研究,为后续的活性研究提供了物质基础。Fifteen lanostane-type triterpenoids were isolated from the mycelia of Ganoderma tsugae through various separation and purification techniques such as medium-high pressure liquid chromatography,preparative thin layer chromatography and gel chromatography.The structures of these compounds were elucidated by nuclear magnetic resonance(NMR)and mass spectrometry(MS).They were identified as:(22S,24E)-3-oxo-15α,22β-dihydroxylanosta-7,9(11),24-trien-26-oic acid(1),(22S,24E)-3-oxo-15α-hydroxy-22β-acetoxylanosta-7,9(11),24-trien-26-oic acid(2),(22S,24E)-3-oxo-15α,22β-diacetoxylanosta-7,9(11),24-trien-26-oic acid(3),15-hydroxy-ganoderic acid S(4),ganodermic acid Jb(5),ganoderic acid P(6),ganodermic acid Ja(7),ganoderic acid X(8),ganodermic acid TQ(9),ganoderic acid Me(10),ganoderic acid Mf(11),ganoderic acid Y(12),ganoderic acid Z(13),lanosta-7,9,(11),24-trien-3α-hydroxy-26-oic acid(14)and ganoderic acid S1(15).All of the fifteen compounds were obtained from the fermented mycelia of G.tsugae for the first time.These results enrich the knowledge of the secondary metabolites of Ganoderma fungi,and provide basis for subsequent pharmacological activity studies.

关 键 词：松杉灵芝 液体发酵 次生代谢产物 结构鉴定 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]