高效液相色谱-串联质谱法分析人参配方颗粒中18种人参皂苷成分  

Determination of 18 Ginsenosides in Ginseng Formulation Granules by High Performance Liquid Chromatography-Mass Spectrometry

作　　者：庄玥 黄艳婷[2] 韩毓之 刘亚雄[2] 邱蕴绮[2] Zhuang Yue;Huang Yanting;Han Yuzhi;Liu Yaxiong;Qiu Yunqi(Guangdong University of Technology,Guangzhou 510006,China;Guangdong Institute for Drug Control,Guangzhou 510663,China)

机构地区：[1]广东工业大学,广东广州510006 [2]广东省药品检验所,广东广州510663

出　　处：《广东化工》2024年第9期152-155,共4页Guangdong Chemical Industry

基　　金：广东省医学科学技术研究基金项目(B2021409);广东省中医药局科研项目(20221055)。

摘　　要：建立了高效液相色谱-串联质谱检测人参配方颗粒中18种人参皂苷定性定量的分析方法。样品经80%甲醇超声提取,采用Agilent Poroshell 120 EC-C_(18)色谱柱(2.1 mm×150 mm,2.7μm)分离,以0.1%甲酸乙腈和0.1%甲酸水溶液作为流动相进行梯度洗脱,电喷雾负离子模式,进行预设定多反应监测(sMRM)模式检测。结果显示,18种目标分析物在各自线性范围内线性良好,相关系数均大于0.9970。平均加标回收率为94.5%~105.2%,RSD为1.6%~3.9%。采用本方法对12份样品进行测定,发现4份样品中检测出拟人参皂苷成分。所建方法快速、可靠、灵敏,适用于人参配方颗粒中18种人参皂苷的定性及定量分析。To develop a high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for qualitative and quantitative analysis of 18 ginsenosides in ginseng formulation granules.Samples were extracted by 80%methanol with ultrasound extraction.The ginsenosides were separated on an Agilent Poroshell 120 EC-C18 column(2.1 mm×150 mm,2.7μm)by gradient elution with 0.1%formic acid acetonitril and 0.1%formic acid water solution as mobile phase.A scheduled multiple reaction monitoring(sMRM)in negative ion mode was adopted in mass spectrometry acquisition.As a result,the linear correlation coefficients(r)of the 18 analytes in the linear ranges were more than 0.9970.The mean recoveries were all in the range of 94.5%~105.2%,and the relative standard deviations(RSD)were between 1.6%and 3.9%.Pse F11 was detected in 4 batches(12 batches in total)samples.The method was proved to be rapid,reliable and sensitive,which can be used to qualitative and quantitative analysis of 18 ginsenosides in ginseng formulation granules.

关 键 词：高效液相色谱-串联质谱 人参 人参皂苷 人参配方颗粒 

分 类 号：TQ[化学工程]