一株木质素降解细菌的分离、鉴定与降解条件优化  

Isolation,Identification and Optimization of Degradation Conditions of Lignin Degrading Strain

作　　者：张亚莉 王敬红 洪艳华[3] 晏磊[1] 章圣龙 崔宗均[4] 王伟东[1,2,3] Zhang Yali;Wang Jinghong;Hong Yanhua;YanLei;Zhang Shenglong;Cui Zong Jun;Wang Weidong(Northeast Plain Agricultural Green Low Carbon Key Laboratory of the Ministry of Agriculture and Rural Affairs/College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319;College of Life Science,Northeast Forestry University;College of Agronomy,Heilongjiang Bayi Agricultural University;College of Agronomy and Biotechnology,China Agricultural University;Heilongjiang GuoHong Environmental Co.,LTD)

机构地区：[1]农业农村部东北平原农业绿色低碳重点实验室/黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]东北林业大学生命科学学院 [3]黑龙江八一农垦大学农学院 [4]中国农业大学农学院 [5]黑龙江国宏节能环保有限公司

出　　处：《黑龙江八一农垦大学学报》2024年第4期71-79,共9页journal of heilongjiang bayi agricultural university

基　　金：国家自然科学基金(U22A20444,32170132);黑龙江省揭榜挂帅科技攻关项目(2022ZXJ08B01);黑龙江八一农垦大学三纵计划重点项目(ZDZX202105)。

摘　　要：高效降解和转化木质素的微生物资源对于秸秆类生物质资源化利用至关重要。通过梯度稀释和苯胺蓝脱色法从木质素降解复合菌系中筛选出木质素降解细菌,经16S rRNA和生理生化试验将菌株归类为Pseudomonas guguanensis。采用单因素、Plackett-Burman和响应面分析的方法确定菌株最佳培养条件。单因素试验表明:木质素添加量、装液量、pH为显著影响因子;响应面试验确定最佳培养条件:装液量为28mL·100 mL^(-1)、木质素添加量为0.6g·L^(-1) pH为7.4。在此条件下,木质素降解率为29.1%,比优化前提高了16.4%,研究丰富了降解木质素的微生物资源。Microbial resources that can efficiently degrade and transform lignin are very important for the utilization of straw biomass resources.The lignin degrading bacteria were screened from the lignin degrading complex strains by gradient dilution and aniline blue decolorization.The strain was classified as Pseudomonas guguanensis by 16S rRNA and physiological and biochemical tests.Single factor,Plackett-Burman and response surface analysis were used to determine the optimal culture conditions.Single factor test showed that lignin addition,liquid loading and pH were significant factors.The optimal culture conditions were determined by response surface test:liquid loading was 28 mL·100 mL^(-1),lignin addition was 0.6 g·L^(-1)and pH was 7.4.Under this condition,the degradation rate of lignin was 29.1%,which was 16.4%higher than that before optimization.It enriched the microbial resources for lignin degradation.

关 键 词：木质素 细菌 降解条件优化 假单胞菌 

分 类 号：Q939.9[生物学—微生物学]