厌氧段光照策略对A/O模式下菌藻共生系统性能的影响  

Influence of illumination strategy in anaerobic stage on the performance of bacterial-algal symbiosis system in A/O mode

作　　者：李冬[1] 公为敏 李帅 柴晨旭 张杰[1,2] LI Dong;GONG Wei-min;LI Shuai;CHAI Chen-xv;ZHANG Jie(Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering,Beijing University of Technology,Beijing 100124,China;State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China)

机构地区：[1]北京工业大学,水质科学与水环境恢复工程北京市重点实验室,北京100124 [2]哈尔滨工业大学城市水资源与水环境国家重点实验室,黑龙江哈尔滨150090

出　　处：《中国环境科学》2024年第8期4336-4346,共11页China Environmental Science

基　　金：北京高校卓越青年科学家计划项目(BJJWZYJH 01201910005019);北京博士后基金项目(2022-ZZ-131)。

摘　　要：设置4组以厌氧/好氧(A/O)模式运行的序批式反应器(SBR),其中R1厌氧段为8000lx的光照;R^(2)、R3和R4将厌氧段划分为A1和A_(2),A1段无光照,A_(2)段的光强分别为0、3000和5000lx,探究不同厌氧段光照策略对菌藻共生系统性能的影响.研究结果显示,在厌氧后期添加弱光照的R3和R4中可以快速富集微藻,这使其形成的菌藻共生颗粒结构致密,沉降性能良好,碳氮磷去除效果更佳,并且分泌出更多的胞外聚合物(EPS).其中R3除磷效果最好,总磷(TP)去除率达到95.87%.典型周期分析可知,弱光条件下厌氧末期的释磷量与叶绿素a(Chl-a)含量成正比,R3的厌氧释磷能力最强,其厌氧末磷浓度达到43.73mg/L,在A_(2)段形成的高磷环境能够刺激微藻生长,其Chl-a含量在运行后期达到6.13mg/gVSS.高通量测序显示,R3的原核生物中成功富集了以Candidatus_Accumulibacter为核心的聚磷菌(PAOs),丰度达到11.18%;真核生物中微藻的总丰度达到84.29%,其中Halochlorella丰度高达42.85%,说明在厌氧后期的高磷环境中添加弱光照(3000lx)能够在不影响PAOs生长的条件下同步富集微藻,形成了以微藻和PAOs为核心的共生颗粒,为污水深度处理提供技术支持.In order to investigate the effects of different illumination strategies in the anaerobic stage on the bacterial-algal symbiotic system,four groups of sequence batch reactors(SBR)were set up to operate in an anaerobic/aerobic(A/O)mode,in which the anaerobic stage of R1 was illuminated with 8000lx;R^(2),R3 and R4 divided the anaerobic stage into A1 and A_(2).There is no illumination in the A1stage,and the illumination intensity in the A_(2)stage respectively are 0,3000,5000lx.The results of this research revealed that R3 and R4 with weak illumination added at the late anaerobic stage could rapidly enriched the microalgae,which led to the formative bacterial-algal symbiotic granular with dense structure,good settling performance,better carbon,nitrogen and phosphorus removal,and the secretion of more extracellular polymers(EPS).Among them,R3 had the best effect of phosphorus removal,and the removal rate of total phosphorus(TP)reached 95.87%.According to the analysis of typical cycle,the phosphorus release at the end of anaerobic stage was proportional to the chlorophyll a(Chl-a)content under weak illumination condition;the ability of R3 to release phosphorus is the strongest,and its phosphorus concentration at the end of anaerobic stage reaching 43.73mg/L;the high-phosphorus environment formed in the A_(2) stage was able to stimulate the growth of microalgae,and its Chl-a content reached 6.13mg/gVSS at the late stage of the operation.High-throughput sequencing showed that the prokaryotic of R3 were successfully enriched phosphorus accumulating bacteria(PAOs)centered on Candidatus_Accumulibacter with an abundance of 11.18%;the total abundance of microalgae in the eukaryotes reached 84.29%,of which the abundance of Halochlorella was as high as 42.85%,suggesting that the addition of weak illumination(3000lx)to the high-phosphorus environment in the late anaerobic stage could stimulate the growth of microalgae without affecting the growth of the PAOs,thus form a symbiotic granular with microalgae and PAOs as the core,wh

关 键 词：菌藻共生 颗粒污泥 光照策略 聚磷菌 高通量测序 

分 类 号：X703.1[环境科学与工程—环境工程]