新型连续流系统中Anammox颗粒粒径控制策略研究  

Study on granule size control strategies in a novel continuous flow system for Anammox

作　　者：李冬[1] 李崧岳 董怡雯 蒋鹏飞 李帅 曾辉平[1] 张杰[1,2] LI Dong;LI Song-yue;DONG Yi-wen;JIANG Peng-fei;LI Shuai;ZENG Hui-ping;ZHANG Jie(Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering,Beijing University of Technology,Beijing 100124,China;State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China)

机构地区：[1]北京工业大学,水质科学与水环境恢复工程北京市重点实验室,北京100124 [2]哈尔滨工业大学,城市水资源与水环境国家重点实验室,黑龙江哈尔滨150090

出　　处：《中国环境科学》2024年第8期4355-4365,共11页China Environmental Science

基　　金：北京高校卓越青年科学家计划项目(BJJWZYJH01201910005019)。

摘　　要：以具有自主专利的新型大/小双室自回流连续流反应器为平台,探究基于粒径回流的Anammox颗粒污泥粒径调控策略及调控机制.粒径回流策略通过改变内循环的位置,将不同粒径的污泥引入高基质浓度和高机械剪切区域进行粒径调控.结果表明,粒径回流策略能够提高Anammox颗粒粒径,并将其控制在合适的范围内.这一策略还增强了系统的污泥浓度、颗粒沉降性能和脱氮性能.其中大颗粒回流策略综合提升效果最佳,维持颗粒粒径在0.5~1.6mm范围内,中值粒径为948μm,污泥体积指数(SVI)为35.6mL/g,总无机氮(TIN)去除率为88.59%.胞外聚合物(EPS)分析和分层实验表明,紧密结合EPS(TB-EPS)中的蛋白质(PN)在粒径调控中起着关键作用,高浓度的外层污泥PN主导了颗粒的沉降性能,而内层紧实污泥的TB-EPS中的PN则增强了颗粒的结构强度.高通量测序结果表明,粒径循环策略能够大幅提高系统内厌氧氨氧化菌(AnAOB)Candidatus Kuenenia的丰度,在大颗粒回流时达到60.24%的峰值,并且AnAOB主要分布在颗粒污泥内层.This study,employed a novel,patented twin-reactor self-recirculating system to explore the granule size control strategies and its controlling mechanisms of anaerobic ammonium oxidation(Anammox)granular sludge based on granules size recirculation approach.By adjusting internal recirculation locations,the strategy aimed to control the size of granules through introducing sludge of varying sizes into regions with high substrate concentration and mechanical shear.The result demonstrated that this strategy effectively increased granule size,and maintained it within an appropriate range.Furthermore,it enhanced sludge concentration,settling characteristics,and nitrogen removal performance.Specifically,recirculating larger granules proved to be highly effective,keeping the size of granular sludge within the optimal range of 0.5~1.6mm,with a median size of 948μm,SVI of 35.6mL/g,and an enhanced total inorganic nitrogen(TIN)removal efficiency of 88.59%.Analysis of extracellular polymeric substances(EPS)revealed that proteins(PN)in the tightly bound EPS(TB-EPS)played a crucial role in granule size control.Stratification experiments revealed that PN in the outer layer of the sludge primarily influenced settleability,while PN in the TB-EPS of the inner layer strengthens the granule structure.High-throughput sequencing showed a significant increase in the abundance of anammox bacteria(AnAOB)Candidatus Kuenenia,mainly located in the inner layers of the sludge granules.These findings have significant implications for the engineering application of anammox granular sludge technology.

关 键 词：厌氧氨氧化 颗粒污泥 连续流 粒径控制 粒径回流 

分 类 号：X703.1[环境科学与工程—环境工程]