硝酸盐异化还原为铵耦合厌氧氨氧化处理含氮废水  

Treatment of nitrogen-containing wastewater by dissimilatory nitrate reduction to ammonium coupled with anaerobic ammonium oxidation

作　　者：赵硕 汪超 杨蒙 乔森[1] ZHAO Shuo;WANG Chao;YANG Meng;QIAO Sen(Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education,China),School of Environmental Science and Technology,Dalian University of Technology,Dalian 116024,China;Dalian Dongtai Organic Waste Treatment Co.,Ltd,Dalian 116000,China)

机构地区：[1]大连理工大学环境学院,工业生态与环境工程教育部重点实验室,辽宁大连116024 [2]大连东泰有机废物处理有限公司,辽宁大连116000

出　　处：《中国环境科学》2024年第8期4389-4399,共11页China Environmental Science

基　　金：国家自然科学基金项目(22176026)。

摘　　要：为探究厌氧氨氧化(Anammox)细菌同时去除废水中铵(NH_(4)^(+))和硝酸盐(NO_(3)^(-))的可行性及长期去除效果,基于Anammox菌的内源硝酸盐异化还原为铵过程,进行了批式实验、抑制剂实验、同位素实验以及长期反应器实验.结果表明,在MLVSS为4500mg/L的批式实验中,经12h反应后NH_(4)^(+)和NO_(3)^(-)被同时去除并生成了N_(2),总氮去除率为93.49%.在乙炔(抑制Anammox反应中铵和一氧化氮生成肼的过程)抑制实验中,检测到17.01mg N/L的NH_(4)^(+),Anammox菌对于NO_(3)^(-)去除的贡献率为85.88%.当加入青霉素G(抑制异养反硝化菌活性)后,NO_(3)^(-)仍可以被去除.在^(14)NH_(4)^(+)和^(15)NO_(3)^(-)同位素示踪实验中一直能够检测到^(29)N_(2)和^(3)0N_(2)的生成,前者是后者含量的1.41倍.在长期实验中,反应器稳定运行阶段平均出水NH_(4)^(+)和NO_(3)^(-)浓度分别为1.18和2.86mg N/L,平均总氮去除率为92.26%.由此可见,Anammox菌通过内源硝酸盐异化还原为铵耦合常规Anammox过程,可以长期稳定地同时去除废水中NH_(4)^(+)和NO_(3)^(-).To investigate the feasibility and long-term performance of simultaneous removal of ammonium(NH_(4)^(+))and nitrate(NO_(3)^(-))in wastewater by anaerobic ammonia oxidation(anammox)bacteria,batch,inhibitor,isotopic and long-term reactor experiments were conducted based on the intracellular carbon-dependent dissimilatory nitrate reduction to ammonium(DNRA)of anammox bacteria.In 12h batch experiments,NH_(4)^(+)and NO_(3)^(-)were simultaneously converted to N_(2) with anammox biomass concentration of 4500mg/L,and the total nitrogen removal efficiency was 93.49%.In the experiment of acetylene inhibition(inhibiting the process of generating hydrazine from NH_(4)^(+)and nitric oxide in the anammox reaction),17.01mg N/L of NH_(4)^(+)was detected,and the contribution rate of anammox bacteria to NO_(3)^(-)removal was 85.88%.After the addition of penicillin G(an inhibitor for most heterotrophic denitrifying bacteria),NO_(3)^(-)could still be removed.Both ^(29)N_(2) and ^(30)N_(2) was always detected from the very beginning in the ^(15)N isotope tracing experiment,and the former was 1.4 folds higher than the latter.In the long-term experiment,the average NH_(4)^(+)and NO_(3)^(-)concentrations of effluent were 1.18 and 2.86mg N/L respectively,reaching an average total nitrogen removal efficiency of 92.26%.These results suggested that NH_(4)^(+)and NO_(3)^(-)could be simultaneously removed by endogenous DNRA coupled with normal anammox metabolism,further promoting the application of anammox-based processes for nitrogen-contained wastewater removal.

关 键 词：厌氧氨氧化 硝酸盐异化还原为铵 铵 硝酸盐 

分 类 号：X703[环境科学与工程—环境工程]