不同碳源反硝化脱氮性能及微生物群落研究  

Study on Nitrogen Removal Efficiency of Denitrification and Microbial Community Structure under Different Carbon Sources

作　　者：韩丰泽 杨小俊 吕凤[1] 石胜宇 王莎莎 龙军 HAN Fengze;YANG Xiaojun;LYU Feng;SHI Shengyu;WANG Shasha;LONG Jun(School of Environmental Engineering,Wuhan Textile University,Wuhan 430200,China;Engineering Research of Center Clean Production of Textile Dyeing and Printing,Ministry of Education,Wuhan 430200,China)

机构地区：[1]武汉纺织大学环境工程学院,湖北武汉430200 [2]纺织印染清洁生产教育部工程研究中心,湖北武汉430200

出　　处：《环境科学与技术》2024年第6期171-178,共8页Environmental Science & Technology

摘　　要：针对污水处理厂深度处理时碳源选择困难和投加量成本高的问题,该研究利用反硝化生物过滤器研究不同碳源对污染物的去除效果,考察了3种碳源(乙酸钠、乙醇、甲醇)在不同水力停留时间(HRT)、碳氮比(C/N)、进水NH_(4)^(+)-N浓度下的脱氮性能和反应器不同高度处的微生物群落结构。结果表明:在低C/N条件下,随着HRT的增加,以乙酸钠、乙醇、甲醇为碳源的3个系统的出水NH_(4)^(+)-N和NO_(3)^(-)-N去除效率逐渐升高,3个系统中甲醇系统的NO_(2)^(-)-N的积累程度最低。随着C/N的逐渐增加,3个系统出水NH_(4)^(+)-N和NO_(3)^(-)-N的浓度都逐渐降低。乙酸钠和甲醇系统分别在C/N为6和5时出水TN浓度<1.5 mg/L,满足地表水Ⅳ类水质标准。随着进水NH_(4)^(+)-N浓度升高,NO_(2)^(-)-N积累程度增加,从而导致出水TN浓度升高。碳源的不同导致门水平下3个系统的优势门丰度明显不同,属水平下差异性则更加明显,反硝化菌的总相对丰度顺序为乙酸钠系统>乙醇系统>甲醇系统。研究结果可为污水处理厂碳源的选择和投加量提供理论参考。In order to solve the problems of difficult selection of carbon sources and high cost of dosage in advanced treatment of wastewater treatment plant,denitrification biological filter was used to explore the denitrification perfor⁃mance of different carbon sources.In this study,the denitrification performance of three carbon sources(sodium acetate,ethanol and methanol)at different hydraulic retention time(HRT),nitrogen ratio(C/N)and influent NH_(4)^(+)-N concentration and the microbial community structure at different heights of the reactor were investigated.The results showed that under the condition of low carbon nitrogen ratio,as the HRT increased,the removal efficiency of NH_(4)^(+)-N and NO_(3)^(-)-N in the effluent of three systems with sodium acetate ethanol and methanol as carbon sources gradually increased,and the accumulation of NO_(2)^(-)-N in methanol system was the lowest in the three systems.With the gradual increase of carbon to C/N,the con⁃centration of NH_(4)^(+)-N and NO_(3)^(-)-N in the three systems gradually decreased.The effluent TN concentration of sodium ace⁃tate and methanol system was less than 1.5 mg/L when C/N was 6 and 5,which met the ClassⅣof Environmental Quality Standard for Surface Water.With the increase of influent NH_(4)^(+)-N concentration,it led to the increase of NO_(2)^(-)-N accu⁃mulation degree,resulting in the increase of effluent TN concentration.Carbon sources led to significant differences in the abundance of the three systems at the phylum level.The differences at the genus level were more obvious,and the total relative abundance of denitrifying bacteria was in the order of sodium acetate system>ethanol system>methanol system.The results of this study can provide a theoretical reference for the selection of carbon source and the use of dosage in waste⁃water treatment plants.

关 键 词：污水深度处理 反硝化生物过滤器 3种碳源 微生物群落结构 

分 类 号：X703[环境科学与工程—环境工程]