工业大麻ZF-HD转录因子的鉴定及表达分析  

Identification and expression analysis of ZF-HD transcription factors of hemp

作　　者：翟莹[1,2] 高双 王志刚[1,2] 赵明[2,3,4] 祁宏英[1,2] 马天意 张军 李珊珊[1,2] ZHAI Ying;CAO Shuang;WANG Zhigang;ZHAO Ming;QI Hong-ying;MA Tian-yi;ZHANG Jun;LI Shan-shan(College of Life Science and Agro-Forestry,Qiqihar University,Qiqihar,Heilongjiang 161006,China;Engineering Research Center of Hemp and Hemp Products in Cold Regions,Ministry of Education,Qiqihar University,Qiqihar,Heilongjiang 161006,China;Institute of Chemistry and Chemical Engineering,Qiqihar University,Qiqihar,Heilongjiang 161006,China;Technology Innovation Center of Industrial Hemp for State Market Regulation,Qiqihar University,Qiqihar,Heilongjiang 161006,China;Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences,Qiqihar,Heilongjiang 161005,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔大学寒区麻及制品教育部工程研究中心,黑龙江齐齐哈尔161006 [3]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006 [4]齐齐哈尔大学国家市场监管技术创新中心(工业大麻),黑龙江齐齐哈尔161006 [5]黑龙江省农业科学院畜牧兽医分院,黑龙江齐齐哈尔161005

出　　处：《西南农业学报》2024年第6期1188-1195,共8页Southwest China Journal of Agricultural Sciences

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(145109506);高教强省科研专项科技成果产业化攻关项目(135209264)。

摘　　要：【目的】对工业大麻ZF-HD(Zinc finger-homeodomain)转录因子进行鉴定和表达模式分析,为ZF-HD功能研究及工业大麻遗传性状的改良提供理论参考。【方法】对5个工业大麻ZF-HD转录因子的基因结构、蛋白理化性质、蛋白系统进化关系和启动子顺式作用元件进行生物信息学分析,通过实时荧光定量PCR(qRT-PCR)对基因表达量进行检测。【结果】5个CsZF-HD基因分布于大麻基因组的2条染色体上,其编码序列长度为783~1185 bp,编码260~394个氨基酸残基,蛋白分子量为27.95~43.33 ku,理论等电点为7.46~8.87。它们编码的蛋白均含有1个保守的ZF-HD_dimer结构域,均为不稳定的亲水性蛋白且定位于细胞核中。ZF-HD蛋白家族分为3个亚族(A、B和C),CsZF-HD1和CsZF-HD2属于A亚族,CsZF-HD3属于C亚族,CsZF-HD4和CsZF-HD5属于B亚族。CsZF-HD1和CsZF-HD2在茎和叶中的表达量相差不明显,在根中表达量最低;CsZF-HD3在茎中的表达量最高,叶中表达量最低;CsZF-HD4在叶中的表达量最高,根中表达量最低;CsZF-HD5在根中表达量最高,茎中表达量最低。5个CsZF-HD基因均对盐和干旱胁迫响应,其中CsZF-HD1和CsZF-HD3在盐和干旱胁迫下表达量升高最明显。5个CsZF-HD基因启动子序列中均含有不同种类和数量的逆境相关顺式作用元件。【结论】ZF-HD转录因子可能在工业大麻应对盐和干旱胁迫时发挥转录调控作用。【Objective】The ZF-HD(Zinc finger-homeodomain)transcriptional factors of hemp were identified and their expression patterns were analyzed to provide theoretical reference for ZF-HD function research and improvement of hemp genetic traits.【Method】The gene structures,protein physicochemical properties,protein phylogenetic relationships and promoter cis-elements of five ZF-HD transcription factors in hemp were analyzed by bioinformatics,and the gene expression levels were analyzed by real-time fluorescent quantitative PCR(qRT-PCR).【Result】Five CsZF-HD genes were distributed on two chromosomes of hemp genome.Their coding sequence lengths were 783-1185 bp,encoding 260-394 amino acids residues.The molecular weights of five CsZF-HD proteins were 27.95-43.33 ku,and the theoretical isoelectric points were 7.46-8.87.Five CsZF-HD proteins all contained a conserved ZF-HD_dimer domain and were unstable hydrophilic proteins localized in the nucleus.The ZF-HD protein family was divided into three subfamilies(A,B and C).CsZF-HD1 and CsZF-HD2 belong to the A subgroup,CsZF-HD3 belongs to the C subgroup,and CsZF-HD4 and CsZF-HD5 belong to the B subgroup.The expression levels of CsZF-HD1 and CsZF-HD2 in stems and leaves were not significantly different,but the expression levels in roots were the lowest.The expression level of CsZF-HD3 was the highest in stems and the lowest in leaves.The expression of CsZF-HD4 was the highest in leaves and the lowest in roots.The expression level of CsZF-HD5 was the highest in roots and the lowest in stems.All the five CsZF-HD genes were responsive to salt and drought stresses,and the expression levels of CsZF-HD1 and CsZF-HD3 were most significantly increased under salt and drought stresses.The promoters of five CsZF-HD genes contained different types and quantities of stress-related cis-elements.【Conclusion】ZF-HD transcription factors may play a role in the transcriptional regulation of hemp in response to salt and drought stresses.

关 键 词：工业大麻 ZF-HD转录因子 生物信息学分析 干旱胁迫 盐胁迫 

分 类 号：S563.3[农业科学—作物学]