水稻纹枯病菌AG-Bb中真菌病毒多样性研究  

Fungal virus diversity in rice sheath blight pathogen AG-Bb

作　　者：陈宁伟 罗丽 张艳[2] 邱润霜 杨应美 赵紫芳 董文汉[1] 杨根华[1] CHEN Ning-wei;LUO Li;ZHANG Yan;QIU Run-shuang;YANG Ying-mei;ZHAO Zi-fang;DONG Wen-han;YANG Gen-hua(State Key Laboratory of Biological Resources Protection and Utilization,Yunnan Agricultural University,Kunming 650201,China;Wenshan Branch of Yunnan Tobacco Company,Wenshan,Yunnan 663099,China)

机构地区：[1]云南农业大学云南生物资源保护与利用国家重点实验室,昆明650201 [2]云南省烟草公司文山州公司,云南文山663099

出　　处：《西南农业学报》2024年第6期1273-1280,共8页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31960525、31160352、31360423);中央引导地方项目(202207AA110005)。

摘　　要：【目的】探明云南水稻纹枯病菌AG-Bb融合亚群的病毒种类,筛选可有效降低水稻纹枯病菌致病力的真菌病毒,为防治水稻纹枯病提供生防病毒资源。【方法】使用二代测序技术(NGS),鉴定水稻纹枯病菌AG-Bb融合亚群菌株8-Q-36、2-R-11和2-Q-13的病毒种类,利用利巴韦林-高温尖端脱毒法筛选具有降低寄主致病力的病毒。【结果】通过比对分析菌株8-Q-36、2-R-11和2-Q-13序列,发现所得病毒可归类于6个真菌病毒科:双分体病毒科(Partitiviridae)、内源体病毒科(Endornaviridae)、镰刀菌病毒科(Fusariviridae)、线粒体病毒科(Mitoviridae)、横纹病毒科(Rhabdoviridae)、全病毒科(Totiviridae),以及部分未分类病毒。与原始菌株8-Q-36相比,脱毒菌株8-Q-36-1的菌落形态更为蓬松,菌核有明显的色素沉着,且致病力明显增强。经双链RNA(dsRNA)提取检测发现,脱毒菌株8-Q-36-1中最大的一条≥10 kb的dsRNA缺失,由此可判定该dsRNA能够降低寄主致病力。经NCBI比对,发现该dsRNA的部分序列为RdRp序列,且与内源体病毒科的RdRp序列具有同源性,与相似性最高的病毒RsEV6序列的一致性为56.02%,因此菌株8-Q-36的分离物可能是一种新病毒,暂命名为RsEV11。【结论】水稻纹枯病菌AG-Bb融合亚群菌株8-Q-36、2-R-11和2-Q-13具有丰富的病毒多样性,其中菌株8-Q-36中存在能够降低水稻纹枯病菌致病力的生防病毒分离物。本研究首次在双核丝核菌属(Ceratobasidium spp.)中发现具有生防价值的真菌病毒。【Objective】The purpose of the experiment was to explore the virus species of Rhizoctonia solani AG-Bb fusion subgroup in Yunnan,screen for fungal viruses that could effectively reduce the pathogenicity of R.solani,and provided biocontrol virus resources for the prevention and control of R.solani.【Method】Next-generation sequencing(NGS)was used to detect the virus species in R.solani AG-Bb fusion subgroup strains 8-Q-36,2-R-11 and 2-Q-13,and identif viruses that could reduce host pathogenicity by the ribavirin high-temperature tip detoxification method.【Result】The results of sequence analysis of strains 8-Q-36,2-R-11 and 2-Q-13 were compared to six mycoviridae,Partitiviridae,Endornaviridae,Fusariviridae,Mitoviridae,Rhabdoviridae,Totiviridae,and some unclassified viruses.Compared with the original strain 8-Q-36,the colony morphology of the detoxified strain 8-Q-36-1 was more fluffy,the sclerotia exhibited obvious pigmentation,and the pathogenicity was significantly enhanced.Double-stranded RNA(dsRNA)extraction and detection showed that the largest≥10 kb dsRNA deletion in the detoxified strain 8-Q-36-1,which led to the determination that the dsRNA could reduce host pathogenicity.After NCBI comparison,it was found that part of the sequence of the dsRNA was RdRp sequence,and it had homology with the RdRp sequence of the endosomic viridae family,and the identity with the sequence of RsEV6,the virus with the highest similarity,was 56.02%,so the isolate of strain 8-Q-36 might be a new virus,tentatively named RsEV11.【Conclusion】The 8-Q-36,2-R-11 and 2-Q-13 strains of R.solani AG-Bb fusion subgroup have rich viral diversity,among which biocontrol virus isolates capable of reducing the pathogenicity of R.solani are present in strain 8-Q-36.In the study,fungal virus with biocontrol value are indentified for the first time in the genus Ceratobasidium spp.

关 键 词：水稻纹枯病菌 AG-Bb融合亚群 病毒多样性 双链RNA 脱毒 低毒力 

分 类 号：S435.111.42[农业科学—农业昆虫与害虫防治]