Mito-TEMPO对猪精子冷冻保存效果的研究  

Spatiotemporal evolution characteristics and driving factors of production of major oil crops in China from 2000 to 2020

作　　者：刘娟 许春荣 刘念 张争光 赵文婧 赵欢欢 许鹏 刘德玉 许常龙[4] 胡传活[1,2] LIU Juan;XU Chun-rong;LIU Nian;ZHANG Zheng-guang;ZHAO W en-jing;ZHAO Huan-huan;XU Peng;LIU De-yu;XU Chang-long;HU Chuan-huo(College of Animal Science and Technology,Guangxi University,Nanning 530004,China;Guangxi Key Laboratory of Livestock and Poultry Breeding and Disease Prevention and Control,Nanning 530004,China;Guangxi Work Station of Livestock Poultry Breed Improvement,Nanning 530001,China;Nanning Second People's Hospital,Nanning 530031,China)

机构地区：[1]广西大学动物科技学院,南宁530004 [2]广西畜禽繁育与疾病防控重点实验室,南宁530004 [3]广西畜禽品种改良站,南宁530001 [4]南宁市第二人民医院,南宁530031

出　　处：《西南农业学报》2024年第6期1370-1376,共7页Southwest China Journal of Agricultural Sciences

基　　金：广西科技重大专项(桂科AA22096032)。

摘　　要：【目的】筛选最佳的Mito-TEMPO浓度并探究其对猪精子冻后质量、抗氧化能力及线粒体功能的影响,为改善猪精子冷冻保存方法提供参考依据。【方法】在冷冻Ⅰ液中添加0.5、5.0、50.0和500.0μmol/L Mito-TEMPO,通过评估冷冻解冻后各组精子的动力学参数和活率,筛选出适用于猪精液冷冻保存的Mito-TEMPO浓度。检测冷冻解冻后猪精子的质膜完整率、顶体完整率、DNA碎片指数(DFI)、活性氧(ROS)水平、总抗氧化能力(T-AOC)、丙二醛(MDA)含量、过氧化氢酶(CAT)和总超氧化物歧化酶(T-SOD)活性,以及ATP和线粒体呼吸链复合物Ⅰ~Ⅴ含量。【结果】与新鲜组(F组)相比,冷冻解冻后精子的动力学参数和活率显著降低(P<0.05,下同),而添加Mito-TEMPO对冻后精子动力学参数和活率均有不同程度的改善,以50.0μmol/L Mito-TEMPO最有效。与F组相比,冻后猪精子的质膜和顶体完整率、T-AOC及ATP和线粒体呼吸链复合物Ⅰ~Ⅴ含量显著降低,CAT和T-SOD活性也显著降低,DFI、ROS水平、MDA含量显著升高。与冷冻对照组(C组)相比,50.0μmol/L Mito-TEMPO组(M组)中精子的质膜完整率、顶体完整率、T-AOC及ATP和线粒体呼吸链复合物Ⅰ、Ⅱ和Ⅴ含量显著升高,DFI、ROS水平、MDA含量显著降低,CAT和T-SOD活性显著提高。【结论】Mito-TEMPO对猪精子具有冷冻保护作用,且Mito-TEMPO的最佳浓度是50.0μmol/L,可通过提高冷冻解冻后猪精子的抗氧化能力和线粒体功能来改善精子质量。【Objective】The present paper aimed to analyze the spatial-temporal agglomeration changes and driving factors of three major oil crops in China from 2000 to 2020,namely soybean,peanut and rape,so as to provide reference for optimizing the production layout of edible oil crops.【Method】The spatiotemporal agglomeration evolution characteristics and driving factors of soybean,peanut and rape production were analyzed by using the gravity shift model,exploratory spatial data analysis,spatiotemporal geographical weighted regression model and other methods.【Result】(i)The yield of soybean was the highest,followed by peanut,and the yield of rape was the lowest.The yield of soybeans showed a significant fluctuation increase,while the yield of rapeseed and peanut showed a slight fluctuation increase.Soybean production was mainly distributed in Heilongjiang,Inner Mongolia,Henan and Anhui.Peanut production was mainly distributed in Shandong,Henan and Guangdong.Rape production was mainly distributed in Sichuan,Hubei and Hunan.(ii)From 2000 to 2020,the yield agglomeration degree of three oil crops showed a weakening trend,and the high-high accumulation areas of soybean yield were in Heilongjiang and Jilin,and the low-low accumulation area was in Xinjiang;The high-high accumulation areas of peanut yield were in Shandong,Henan and Anhui,and the low-low accumulation area was in Xinjiang;The high-high accumulation areas of rape yield were in Hubei,Anhui and Jiangxi,and the low-low accumulation area was in Inner Mongolia.The production center of soybean moved to the northwest from Tanghai county in Hebei province to Ningcheng county in Inner Mongolia.The production center of peanut was located in Henan province and was moving towards the northwest of Henan province;The production center of rapeseed shifted to the southwest from Nanzhang county in Hubei province to Fengjie county in Chongqing.(iii)The driving factors of different oil production had different effects in different provinces.Soybean production was mainly affec

关 键 词：Mito-TEMPO 猪 精子 冷冻保存 氧化应激 

分 类 号：S828[农业科学—畜牧学]