pH、葡萄糖和几种阳离子对本溪细鳞鱼精子活力的影响  

Effects of pH, glucose and several cations on spermmotility of Benxi Brachymystax lenok Pallas

作　　者：李卓然 胡雅悦 王茂林 王常安[2] LI Zhuoran;HU Yayue;WANG Maolin;WANG Chang’an(Liaoning Northern Key Laboratory of Applied Biology and Aquaculture of Fish,Dalian Ocean University,Dalian 116023,China;Heilongjiang Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150000,China)

机构地区：[1]大连海洋大学辽宁省北方鱼类应用生物学与增养殖重点实验室,辽宁大连116023 [2]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150076

出　　处：《河北渔业》2024年第8期14-18,46,共6页

基　　金：2021年辽宁省海洋经济发展项目资助;2023年辽宁省教育厅面上项目(JYTMS)。

摘　　要：为给本溪细鳞鱼(Brachymystax lenok Pallas)人工繁殖中最佳受精激活介质的选择提供理论依据,也为细鳞鱼的精子稀释液和保存液的配制提供科学依据,采用平板载片法,研究了pH、葡萄糖和Na^(+)、K^(+)、Ca^(2+)、Mg^(2+)等金属离子以及冷藏时间对本溪细鳞鱼精子活力的影响。结果显示,精子应在超低温条件下进行保存。研究表明,应采用自来水配置,pH为8,且应含有0.5 g/L Na^(+)、0.3 g/L Mg^(2+)、0.3 g/L Ca^(2+)和50 mmol/L葡萄糖,从而达到本溪细鳞鱼人工授精的最佳条件。研究得出,适宜浓度的pH值和葡萄糖、Na^(+)、Mg^(2+)、Ca^(2+)对本溪细鳞鱼精子活力有增强作用。K^(+)会抑制本溪细鳞鱼的精子活力,而Na^(+)是促进本溪细鳞鱼精子活力的主要因素。To provide a theoretical basis for the selection of the best fertilization activation medium in the artificial breeding of Benxi Manchurian trout(Brachymystax lenok Pallas),and also to provide a scientific basis for the preparation of sperm dilution and preservation solution of the fish,the effects of pH,glucose,metal ions such as Na^(+),K^(+),Ca^(2+),Mg^(2+)and cold storage time on sperm motility of Benxi Manchurian trout were studied by plate slide method.The results showed that sperm should be stored at ultra-low temperatures,the activation solution of artificial insemination of Benxi Manchurian trout should be prepared with tap water,pH 8,and contain 0.5 g/L Na^(+),0.3 g/L Mg^(2+),0.3 g/L Ca^(2+)and 50 mmol/L glucose.It was concluded that the appropriate pH value and glucose,Na^(+),Mg^(2+),Ca^(2+)can enhance sperm motility of Benxi Manchurian trout.K^(+)can inhibit the sperm motility of Benxi Manchurian trout.Na^(+)is the main factor promoting sperm motility of Benxi Manchurian trout.

关 键 词：细鳞鱼(Brachymystax lenok Pallas) 精子活力 金属离子 PH 葡萄糖 

分 类 号：S965[农业科学—水产养殖]