紫菀UPLC指纹图谱及化学计量学质量分析评价  

UPLC Fingerprint and Chemometric Quality Analysis Evaluation of Aster Tataricum

作　　者：夏鹏飞 王晓雁 李开斌 Xia Pengfei;Wang Xiaoyan;Li Kaibin(Anshun Food and Drug Inspection Institute,Anshun 561000,China)

机构地区：[1]安顺市食品药品检验所,贵州安顺561000

出　　处：《广东化工》2024年第14期137-139,133,共4页Guangdong Chemical Industry

基　　金：安顺市科技创新平台建设计划项目(安市科平[2017]03号)。

摘　　要：目的:建立紫菀药材UPLC指纹图谱方法,结合化学计量学方法对主要产地紫菀药材质量进行评价。方法:收集主要产地紫菀药材16批次,建立超高效液相色谱(UPLC)指纹图谱方法,并采用中药指纹图谱相似度评价软件数据分析。根据指纹图谱信息结合化学计量学聚类分析法(clustering analysis,CA)、主成分分析法(principal component analysis,PCA)及偏最小二乘判别法(Partial Least Squares Discriminant Analysis,PLS-DA)对其质量进行综合评价。结果:紫菀药材的指纹图谱有17个共有峰,相似度结果显示16批紫菀药材相似度大于0.95,一批为0.849,共指认了其中的8个共有峰,即峰2、峰6、峰7、峰9、峰10、峰13、峰17、峰19,分别为5-咖啡酰奎宁酸、隐绿原酸、咖啡酸、1,3-O-二咖啡酰奎宁酸、阿魏酸、1,5-O-二咖啡酰奎宁酸、槲皮素、山柰素;16批紫菀药材通过聚类分析以组间距为15可聚为2类,主成分分析共提取了2类主成分,采用因子综合得分评价了3个产地紫菀的质量,PLS-DA模型的变量重要性投影VIP值共筛选出2个差异性成分。结论:建立的指纹图谱可以反映紫菀的化学成分分布,能为紫菀的整体质量评价提供参考,结合化学计量学方法全面地分析了3个产地、不同批次紫菀的质量状况,为紫菀药材质量现状分析提供数据支持。Objective:to establish UPLC fingerprint method radixa asteriseris and to evaluate the quality of radix asteris from the main producing areas by using the Chemometrics method.Methods:16 batches of radix asteris were collected and the UPLC fingerprint was established,and the High-performance liquid chromatography data were analyzed by similarity evaluation software.The quality of the fingerprint was evaluated by Chemometrics clustering Analysis(CA),principal component Analysis(PCA)and Partial Least Squares Discriminant Analysis(PLS-DA).The results showed that there were 17 common peaks in the fingerprint of radix aster Aster tataricus.The similarity of 16 batches of radix aster was greater than 0.95 and that of one batch was 0.849,peak 2,Peak 6,Peak 7,Peak 9,Peak 10,Peak 13,Peak 17,peak 19 are 5-caffeylquinic acid,cryptochlorogenic acid,caffeic acid,artichoke,ferulic acid,1,16 batches of radix aster were clustered into 2 classes by cluster analysis,and 2 classes of principal components were extracted by principal component analysis,the quality of Aster from 3 areas was evaluated by the factor comprehensive score.The VIP value of the variable importance projection of PLS-DA model screened out 2 different components.Conclusion:the established fingerprint can reflect the chemical composition distribution of Aster,and can provide reference for the overall quality evaluation of Aster,the quality of radix asteris from 3 different habitats and batches was analyzed by Chemometrics method,which provided data support for the quality analysis of Radix Asteris in Aster tataricus.

关 键 词：紫菀 指纹图谱 化学计量学 质量评价 

分 类 号：TQ[化学工程]